Immunohistochemistry (Frozen sections) abreview for Anti-HA tag antibody [HA.C5]

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (frozen liver sections)
Permeabilization
Yes - Triton X 0.3% - 10 min
Specification
frozen liver sections
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/400

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The antibody induced unspecific cytoplasmic signals in both positive and negative control
Abcam response
Thank you for submitting an AbReview for ab18181 (Anti-HA tag antibody [HA.C5]). It is unfortunate that the outcome of your experiment using ab18181 is inconclusive. This antibody has not been tested in IHC-Fr before and it was not known if ab18181 is suitable for this application. However, further optimization of the protocol may improve the results obtained.

Abcam user community

Verified customer

Submitted Jun 30 2016

Sign up