Overview

  • Product name

    Anti-HA tag antibody (HRP)
    See all HA tag primary antibodies
  • Description

    Rabbit polyclonal to HA tag (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Human HA tag conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HEK293 (Human embryonic kidney cell line) - expressing HA tag protein; E. coli Positive Control (Escherichia coli ).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 48,55 kDa (predicted molecular weight: 48,55 kDa). Abcam recommends using 3% milk as the blocking agent.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody (HRP) (ab128131) at 1/5000 dilution

    Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) at 10 µg
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48,55 kDa
    Observed band size: 48,55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 150 seconds


    Abcam recommends using 3% milk as the blocking agent.

References

This product has been referenced in:

  • Scholl A  et al. Tracheal expression of Osiris gene family in Drosophila. Gene Expr Patterns 28:87-94 (2018). Read more (PubMed: 29548969) »
  • Seo AY  et al. AMPK and vacuole-associated Atg14p orchestrate µ-lipophagy for energy production and long-term survival under glucose starvation. Elife 6:N/A (2017). WB . Read more (PubMed: 28394250) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (yeast cell lysate)
Specification
yeast cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 26 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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