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I am attempting to find a product that would be used to create a standard curve for a quantitative western. My protein of interest has an HA-tag but cannot really be purified, would product ab13835 be suitable for creating a standard curve in a western?
Asked on Sep 24 2012
Thank you for contacting Abcam regarding ab13835.
This product is the HA peptide used as the immunogen to generate the antibody ab13834. Since it is only 9aa, it cannot be run on a gel to use as a positive control or standard. Instead, I would recommend a purified HA-tagged protein or a cell lysate containing an HA tagged protein, such as ab20896.
I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.
Answered on Sep 24 2012