Key features and details
- Rabbit polyclonal to HABP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HABP2 antibody
See all HABP2 primary antibodies
DescriptionRabbit polyclonal to HABP2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein (His-T7-tag) corresponding to Human HABP2 aa 24-560. (Expressed in E.coli).
FSLMSLLESLDPDWTPDQYDYSYEDYNQEENTSSTLTHAENPDWYYTEDQ ADPCQPNPCEHGGDCLVHGSTFTCSCLAPFSGNKCQKVQNTCKDNPCGRG QCLITQSPPYYRCVCKHPYTGPSCSQVVPVCRPNPCQNGATCSRHKRRSK FTCACPDQFKGKFCEIGSDDCYVGDGYSYRGKMNRTVNQHACLYWNSHLL LQENYNMFMEDAETHGIGEHNFCRNPDADEKPWCFIKVTNDKVKWEYCDV SACSAQDVAYPEESPTEPSTKLPGFDSCGKTEIAERKIKRIYGGFKSTAG KHPWQASLQSSLPLTISMPQGHFCGGALIHPCWVLTAAHCTDIKTRHLKV VLGDQDLKKEEFHEQSFRVEKIFKYSHYNERDEIPHNDIALLKLKPVDGH CALESKYVKTVCLPDGSFPSGSECHISGWGVTETGKGSRQLLDAKVKLIA NTLCNSRQLYDHMIDDSMICAGNLQKPGQDTCQGDSGGPLTCEKDGTYYV YGIVSWGLECGKRPGVYTQVTKFLNWIKATIKSESGF
Database link: Q14520
- IHC-P: Human rectum tissue. WB: Recombinant human HABP2 protein; Pig liver, kidney and small intestine lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232778 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 63 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 3 EGF-like domains.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsProteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments.
Cellular localizationSecreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
- Information by UniProt
- Factor seven activating protease antibody
- Factor seven-activating protease antibody
- Factor VII activating protein antibody
Formalin-fixed, paraffin-embedded human rectum tissue stained for HABP2 using ab232778 at 20µg/mL in immunohistochemical analysis. DAB staining.
Anti-HABP2 antibody (ab232778) at 2 µg/ml + Recombinant human HABP2 protein.
Predicted band size: 63 kDa
All lanes : Anti-HABP2 antibody (ab232778) at 1 µg/ml
Lane 1 : Pig kidney tissue lysate
Lane 2 : Pig intestine tissue lysate
Lane 3 : Pig liver tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 63 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232778 has not yet been referenced specifically in any publications.