Overview

  • Product name

    Anti-HABP2 antibody [EPR14551]
    See all HABP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14551] to HABP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HABP2 aa 350-450. The exact sequence is proprietary.
    Database link: Q14520

  • Positive control

    • Human fetal liver, HepG2, MCF-7 and A549 lysate. Rat liver tissue. HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14551
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 63 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 3 EGF-like domains.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments.
  • Cellular localization

    Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
  • Information by UniProt
  • Database links

  • Alternative names

    • Factor seven activating protease antibody
    • Factor seven-activating protease antibody
    • Factor VII activating protein antibody
    • Factor VII-activating protease antibody
    • FSAP antibody
    • HABP 2 antibody
    • HABP antibody
    • Habp2 antibody
    • HABP2_HUMAN antibody
    • Hepatocyte growth factor activator like protein antibody
    • Hepatocyte growth factor activator-like protein antibody
    • HGFAL antibody
    • Hyaluronan binding protein 2 antibody
    • Hyaluronan-binding protein 2 27 kDa light chain alternate form antibody
    • Hyaluronic acid binding protein 2 antibody
    • PHBP antibody
    • Plasma hyaluronan binding protein antibody
    • Plasma hyaluronan-binding protein antibody
    see all

Images

  • All lanes : Anti-HABP2 antibody [EPR14551] (ab181837) at 1/50000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 lysate
    Lane 3 : MCF-7 lysate
    Lane 4 : A549 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded rat liver tissue sections labeling HABP2 using ab181837 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemical analysis of formaldehyde fixed HepG2 cells labeling HABP2 using ab181837 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution. Counterstain DAPI.

References

This product has been referenced in:

  • Ichimaru S  et al. Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27347945) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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