Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14551] to HABP2
- Suitable for: WB, Flow Cyt, ICC
- Reacts with: Mouse, Rat, Human
Product nameAnti-HABP2 antibody [EPR14551]
See all HABP2 primary antibodies
DescriptionRabbit monoclonal [EPR14551] to HABP2
Tested applicationsSuitable for: WB, Flow Cyt, ICCmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HABP2 aa 350-450. The exact sequence is proprietary.
Database link: Q14520
- ICC: MCF7. Flow Cyt: HepG2. WB: MCF2 and A549 whole cell lysate. Mouse brain and heart lysate. Rate brain and heart lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Predicted molecular weight: 63 kDa.
For unpurified use at 1/10000 - 1/50000.
For unpurified use at 1/100.
FunctionCleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 3 EGF-like domains.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsProteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments.
Cellular localizationSecreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
- Information by UniProt
- Factor seven activating protease antibody
- Factor seven-activating protease antibody
- Factor VII activating protein antibody
Lane 1 : Anti-HABP2 antibody [EPR14551] (ab181837) at 1/10000 dilution (Purified)
Lanes 2-6 : Anti-HABP2 antibody [EPR14551] (ab181837) at 1/10000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : Mouse brain lysate at 20 µg
Lane 4 : Mouse heart lysate at 20 µg
Lane 5 : Rat brain lysate at 1/20 dilution
Lane 6 : Rat heart lysate at 20 µg
Lanes 1-2 & 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Lane 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/50 dilution (2.46 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
ab181837 has been referenced in 2 publications.
- Hong Y et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles 8:1670893 (2019). PubMed: 31632619
- Ichimaru S et al. Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage. Int J Mol Sci 17:N/A (2016). PubMed: 27347945