Overview

  • Product name

  • Description

    Rabbit polyclonal to HACE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human HACE1 aa 333-442.
    Database link: Q8IYU2

  • Positive control

    • IHC-P: Human heart and brain tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab237045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    E3 ubiquitin-protein ligase that may function in cellular proteins degradation. HACE1 has also been shown to be down-regulated in many human tumours and is a putative candidate tumor suppressor.
  • Cellular localization

    Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. A significant portion localizes to the endoplasmic reticulum.
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase HACE1 antibody
    • HACE 1 antibody
    • HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 antibody
    • KIAA1320 antibody

Images

  • Paraffin-embedded human heart tissue stained for HACE1 using ab237045 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human brain tissue stained for HACE1 using ab237045 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab237045 has not yet been referenced specifically in any publications.

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