Overview

  • Product name

    Anti-HACE1 antibody [EPR7962]
    See all HACE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7962] to HACE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HACE1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T and SH-SY5Y cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    Images

    • Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain lysate at 20 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : SH-SY5Y whole cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate at 10 µg

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa

    References

    This product has been referenced in:

    See all 4 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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