Recombinant Anti-HACE1 antibody [EPR7962] (ab133637)
![Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)](https://www.abcam.com/ps/products/133/ab133637/Images/ab133637-256935-anti-hace1-antibody-epr7962-western-blot.jpg "Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7962] to HACE1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HACE1 antibody [EPR7962]
See all HACE1 primary antibodies -
Description
Rabbit monoclonal [EPR7962] to HACE1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HACE1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- 293T and SH-SY5Y cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7962 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
Target
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Relevance
E3 ubiquitin-protein ligase that may function in cellular proteins degradation. HACE1 has also been shown to be down-regulated in many human tumours and is a putative candidate tumor suppressor. -
Cellular localization
Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. A significant portion localizes to the endoplasmic reticulum. -
Database links
- Entrez Gene: 57531 Human
- Entrez Gene: 209462 Mouse
- Entrez Gene: 361866 Rat
- Omim: 610876 Human
- SwissProt: Q8IYU2 Human
- SwissProt: Q3U0D9 Mouse
- SwissProt: D3ZBM7 Rat
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Alternative names
- E3 ubiquitin-protein ligase HACE1 antibody
- HACE 1 antibody
- HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 antibody
- KIAA1320 antibody
Images
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Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)](https://www.abcam.com/ps/products/133/ab133637/Images/ab133637-256934-anti-hace1-antibody-epr7962-western-blot.jpg)
Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)](https://www.abcam.com/ps/products/133/ab133637/Images/ab133637-256933-anti-hace1-antibody-epr7962-western-blot.jpg)
Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)
Lane 1 : HEK293 whole cell lysate
Lane 2 : SH-SY5Y whole cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)](https://www.abcam.com/ps/products/133/ab133637/Images/ab133637-147547-anti-hace1-antibody-epr7962-western-blot.jpg)
Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate at 10 µg
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
![Anti-HACE1 antibody [EPR7962] (ab133637)](https://www.abcam.com/ps/products/133/ab133637/Images/ab133637-6-benefits-of-recombinant-antibodies.png)
Anti-HACE1 antibody [EPR7962] (ab133637)
Datasheets and documents
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Datasheet download
References (6)
ab133637 has been referenced in 6 publications.
- Li S et al. Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells. Oncol Lett 20:280 (2020). PubMed: 33014158
- Kogler M et al. HACE1 Prevents Lung Carcinogenesis via Inhibition of RAC-Family GTPases. Cancer Res 80:3009-3022 (2020). PubMed: 32366477
- El-Naggar AM et al. HACE1 is a potential tumor suppressor in osteosarcoma. Cell Death Dis 10:21 (2019). PubMed: 30622235
- Yu Z et al. Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells. Oncol Lett 17:4361-4368 (2019). PubMed: 30988809
- Goka ET & Lippman ME Loss of the E3 ubiquitin ligase HACE1 results in enhanced Rac1 signaling contributing to breast cancer progression. Oncogene 34:5395-405 (2015). WB ; Human . PubMed: 25659579
- Hollstein R et al. HACE1 deficiency causes an autosomal recessive neurodevelopmental syndrome. J Med Genet 52:797-803 (2015). WB . PubMed: 26424145
