RecombinantRabMAb

Recombinant Anti-HACE1 antibody [EPR7962] (ab133637)

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Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
  • Anti-HACE1 antibody [EPR7962] (ab133637)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7962] to HACE1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Carrier Free

Overview

  • Product name

    Anti-HACE1 antibody [EPR7962]
    See all HACE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7962] to HACE1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human HACE1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T and SH-SY5Y cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
Application notes
Is unsuitable for ICC or IHC-P.

Target

  • Relevance

    E3 ubiquitin-protein ligase that may function in cellular proteins degradation. HACE1 has also been shown to be down-regulated in many human tumours and is a putative candidate tumor suppressor.

  • Cellular localization

    Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. A significant portion localizes to the endoplasmic reticulum.

  • Database links

    • Alternative names

      • E3 ubiquitin-protein ligase HACE1 antibody
      • HACE 1 antibody
      • HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 antibody
      • KIAA1320 antibody

    Images

    • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain lysate at 20 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : SH-SY5Y whole cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 102 kDa
      Observed band size: 102 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
      All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate at 10 µg

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa

    • Anti-HACE1 antibody [EPR7962] (ab133637)
      Anti-HACE1 antibody [EPR7962] (ab133637)

    References (6)

    Publishing research using ab133637? Please let us know so that we can cite the reference in this datasheet.

    ab133637 has been referenced in 6 publications.

    • Li S  et al. Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells. Oncol Lett 20:280 (2020). PubMed: 33014158
    • Kogler M  et al. HACE1 Prevents Lung Carcinogenesis via Inhibition of RAC-Family GTPases. Cancer Res 80:3009-3022 (2020). PubMed: 32366477
    • El-Naggar AM  et al. HACE1 is a potential tumor suppressor in osteosarcoma. Cell Death Dis 10:21 (2019). PubMed: 30622235
    • Yu Z  et al. Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells. Oncol Lett 17:4361-4368 (2019). PubMed: 30988809
    • Goka ET & Lippman ME Loss of the E3 ubiquitin ligase HACE1 results in enhanced Rac1 signaling contributing to breast cancer progression. Oncogene 34:5395-405 (2015). WB ; Human . PubMed: 25659579
    • Hollstein R  et al. HACE1 deficiency causes an autosomal recessive neurodevelopmental syndrome. J Med Genet 52:797-803 (2015). WB . PubMed: 26424145

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