Anti-HADH antibody (ab154088)
Key features and details
- Rabbit polyclonal to HADH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HADH antibody
See all HADH primary antibodies -
Description
Rabbit polyclonal to HADH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human HADH aa 92-314.
Database link: Q16836 -
Positive control
- WB: Mouse liver whole cell lysates. HEK-293T, A431, HeLa, HepG2 whole cell lysate. Rat heart and liver extract. IHC-P: Human colon cancer tissue; ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
|
IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. -
Tissue specificity
Expressed in liver, kidney, pancreas, heart and skeletal muscle. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency) [MIM:231530]. HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death.
Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4) [MIM:609975]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion. -
Sequence similarities
Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3033 Human
- Entrez Gene: 15107 Mouse
- Entrez Gene: 113965 Rat
- Omim: 601609 Human
- SwissProt: Q16836 Human
- SwissProt: Q61425 Mouse
- SwissProt: Q9WVK7 Rat
- Unigene: 438289 Human
see all -
Alternative names
- 3 hydroxyacyl Coenzyme A dehydrogenase antibody
- HAD antibody
- HADH antibody
see all
Images
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Anti-HADH antibody (ab154088) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 34 kDa
10% SDS PAGE -
All lanes : Anti-HADH antibody (ab154088) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell
lysate/extract
Lane 3 : HeLa whole cell
lysate/extract
Lane 4 : HepG2 whole cell
lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
10% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling HADH with ab154088 at 1/250 dilution.
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Immunofluorescence analysis of HeLa cells (fixed with 2% paraformaldehyde/culture medium at 37 °C for 30 min) labeling HADH with ab154088 at 1/500 dilution (green). The image in the right panel is costained with Hoechst 33342 (blue).
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Anti-HADH antibody (ab154088) at 1/1000 dilution + Rat tissue extract at 50 µg
Predicted band size: 34 kDa12% SDS-PAGE
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Anti-HADH antibody (ab154088) at 1/1000 dilution + Rat tissue extract at 50 µg
Predicted band size: 34 kDa12% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab154088 has been referenced in 12 publications.
- Almquist NW et al. No Differences Between 12 Weeks of Block- vs. Traditional-Periodized Training in Performance Adaptations in Trained Cyclists. Front Physiol 13:837634 (2022). PubMed: 35299664
- Almquist NW et al. Effects of Including Sprints in LIT Sessions during a 14-d Camp on Muscle Biology and Performance Measures in Elite Cyclists. Med Sci Sports Exerc 53:2333-2345 (2021). PubMed: 34081058
- Zhang J et al. MiR-103a-3p aggravates renal cell carcinoma by targeting TMEM33. Am J Transl Res 13:12694-12703 (2021). PubMed: 34956484
- Mendham AE et al. Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity. Diabetologia N/A:N/A (2021). PubMed: 33770195
- Fiorentino TV et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378