Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH6.0
Sample
Human Tissue sections (pancreas)
Specification
pancreas
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti rabbit Alexa 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

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Submitted Jun 13 2013

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