Key features and details
- Mouse monoclonal [4A8BG12] to HADHA/HADHB
- Suitable for: ICC, IHC-P, Flow Cyt, IP
- Reacts with: Rat, Cow, Human
- Isotype: IgG1
Product nameAnti-HADHA/HADHB antibody [4A8BG12]
DescriptionMouse monoclonal [4A8BG12] to HADHA/HADHB
Tested applicationsSuitable for: ICC, IHC-P, Flow Cyt, IPmore details
Species reactivityReacts with: Rat, Cow, Human
Tissue, cells or virus. This information is considered to be commercially sensitive.
- HDFn and HL60 cells; HepG2 cell lysate; Human cerebellum tissue.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab110302 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab110302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration. 0.5-2mg protein/10ul antibody|
PathwayLipid metabolism; fatty acid beta-oxidation.
Involvement in diseaseDefects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
Sequence similaritiesBelongs to the thiolase family.
- Information by UniProt
- 3-ketoacyl-CoA thiolase antibody
- 4930479F15Rik antibody
- Acetyl-CoA acyltransferase antibody
Immunocytochemistry analysis using ab110302 at 5µg/ml staining HADHA/HADHB in HDFn cells.
Flow cytometric analysis using ab110302 at 1µg/ml staining HADHA/HADHB in HL60 cells (blue). Isotype control antibody (red).
Detection of ab110302 by Western Blot of Immunprecipitate.
Staining of HADHA/HADHB in 1 mg HepG2 cell lysate/10ul antibody conjugated beads immunoprecipitated using ab110302.
Immunohistochemistry analysis using ab110302 staining HADHA/HADHB in Human cerebellum Formalin-fixed, Paraffin-embedded tissue.
ab110302 has been referenced in 1 publication.
- Margenat M et al. New potential eukaryotic substrates of the mycobacterial protein tyrosine phosphatase PtpA: hints of a bacterial modulation of macrophage bioenergetics state. Sci Rep 5:8819 (2015). PubMed: 25743628