Overview

  • Product name

    Anti-HADHA/HADHB antibody [4A8BG12]
  • Description

    Mouse monoclonal [4A8BG12] to HADHA/HADHB
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HDFn and HL60 cells; HepG2 cell lysate; Human cerebellum tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration. 0.5-2mg protein/10ul antibody

Target

  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease

    Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
  • Sequence similarities

    Belongs to the thiolase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-ketoacyl-CoA thiolase antibody
    • 4930479F15Rik antibody
    • Acetyl-CoA acyltransferase antibody
    • Beta-ketothiolase antibody
    • C77020 antibody
    • ECHA antibody
    • ECHB antibody
    • ECHB_HUMAN antibody
    • GBP antibody
    • HADH antibody
    • Hadhb antibody
    • LCEH antibody
    • LCHAD antibody
    • MGC105338 antibody
    • MGC1728 antibody
    • MGC87480 antibody
    • MS734 antibody
    • MSTP029 antibody
    • MTPA antibody
    • MTPB antibody
    • RGD1560655 antibody
    • TP ALPHA antibody
    • TP-beta antibody
    • Trifunctional enzyme subunit alpha, mitochondrial antibody
    see all

Images

  • Immunocytochemistry analysis using ab110302 at 5µg/ml staining HADHA/HADHB in HDFn cells.
  • Flow cytometric analysis using ab110302 at 1µg/ml staining HADHA/HADHB in HL60 cells (blue). Isotype control antibody (red).
  • Detection of ab110302 by Western Blot of Immunprecipitate.
    Staining of HADHA/HADHB in 1 mg HepG2 cell lysate/10ul antibody conjugated beads immunoprecipitated using ab110302.
  • Immunohistochemistry analysis using ab110302 staining HADHA/HADHB in Human cerebellum Formalin-fixed, Paraffin-embedded tissue.

References

This product has been referenced in:

  • Margenat M  et al. New potential eukaryotic substrates of the mycobacterial protein tyrosine phosphatase PtpA: hints of a bacterial modulation of macrophage bioenergetics state. Sci Rep 5:8819 (2015). Read more (PubMed: 25743628) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2016

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Ms. Arcan Guven

Verified customer

Submitted Sep 03 2014

Question
Answer

Thank you very much for your telephone call this morning. As discussed, to our knowledge I am sorry to confirm that ab110302 or ab110281 have not been tested in rabbit samples. Both these antibodies have been raised against the native human HADHA protein, the sequence of which is on the following protein database link: http://www.uniprot.org/uniprot/P40939 I am sorry I have regrettably not been able to find the rabbit sequence to perform an alignment for you. I can recommend to try this if you have the rabbit sequence? Or if you are able to send the rabbit sequence to me, I can perform the alignment for you. As discussed on the phone, if the alignment is high enough, it is quite likely that the antibody would detect in rabbit. In this case, if the alignment is high I will be able to offer a discount off a future purchase if you buy one of the antibodies now, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If the alignment is high and you are interested in this offer, you would then need to proceed as follows: 1. Let me know that you would like to proceed and test one of the antibodies in rabbit. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering. 2. Purchase the antibody either by phone, fax, or online (www.abcam.com). 3. Test it in rabbit. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. I look forward to hearing from you with the rabbit sequence or the results of the alignment. Please let me know if you have any questions about the offer and I would be happy to help you further. Have a good day.

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