Anti-HADHB antibody [5C3AB7] (ab110301)
Key features and details
- Mouse monoclonal [5C3AB7] to HADHB
- Suitable for: IP, WB, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-HADHB antibody [5C3AB7]
See all HADHB primary antibodies -
Description
Mouse monoclonal [5C3AB7] to HADHB -
Host species
Mouse -
Tested applications
Suitable for: IP, WB, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 and HLM cell lysates; HDFn and HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. -
Clonality
Monoclonal -
Clone number
5C3AB7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC |
Use a concentration of 1 µg/ml.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
Use a concentration of 1 µg/ml. |
Target
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Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3032 Human
- Omim: 143450 Human
- SwissProt: P55084 Human
- Unigene: 515848 Human
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Alternative names
- 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
- 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
- 3 ketoacyl Coenzyme A thiolase antibody
see all
Images
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Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml (20 ug HepG2 cell lysate)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa -
Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml + HepG2 cell lysate
Predicted band size: 51 kDaAs a Western blot antibody, ab110301 works with HepG2 (human) cells.
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Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue). -
Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red).
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As an IP antibody, MS733 works with human samples.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110301 has been referenced in 1 publication.
- Li XX et al. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell 69:480-492.e7 (2018). PubMed: 29395065