Key features and details
- Mouse monoclonal [5C3AB7] to HADHB
- Suitable for: IP, WB, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HADHB antibody [5C3AB7]
See all HADHB primary antibodies
DescriptionMouse monoclonal [5C3AB7] to HADHB
Tested applicationsSuitable for: IP, WB, Flow Cyt, ICCmore details
Species reactivityReacts with: Human
This information is considered to be commercially sensitive.
- HepG2 and HLM cell lysates; HDFn and HeLa cells.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab110301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use a concentration of 1 µg/ml.|
PathwayLipid metabolism; fatty acid beta-oxidation.
Involvement in diseaseDefects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
Sequence similaritiesBelongs to the thiolase family.
- Information by UniProt
- 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
- 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
- 3 ketoacyl Coenzyme A thiolase antibody
Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml (20 ug HepG2 cell lysate)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml + HepG2 cell lysate
Predicted band size: 51 kDa
As a Western blot antibody, ab110301 works with HepG2 (human) cells.
Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red).
As an IP antibody, MS733 works with human samples.
ab110301 has been referenced in 1 publication.
- Li XX et al. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell 69:480-492.e7 (2018). PubMed: 29395065