Overview

  • Product name

  • Description

    Rabbit polyclonal to HADHB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human HADHB.
    Database link: P55084

  • Positive control

    • IHC-P: Human pancreas, heart and liver tissue. ICC/IF: A549 cells. WB: BT-474, SW480, HepG2, HEK-293T and Jurkat cell extracts. Mouse liver, kidney and heart extracts. Rat liver extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab230667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 51 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/100.

Target

  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease

    Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
  • Sequence similarities

    Belongs to the thiolase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
    • 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
    • 3 ketoacyl Coenzyme A thiolase antibody
    • 3 ketoacyl Coenzyme A thiolase of mitochondrial trifunctional protein beta subunit antibody
    • 3-ketoacyl-CoA thiolase antibody
    • Acetyl CoA acyltransferase antibody
    • Acetyl-CoA acyltransferase antibody
    • Beta ketothiolase antibody
    • Beta-ketothiolase antibody
    • ECHB antibody
    • ECHB_HUMAN antibody
    • HADH antibody
    • Hadhb antibody
    • Hydroxyacyl CoA dehydrogenase/3 ketoacyl CoA thiolase/enoyl CoA hydratase (trifunctional protein), beta subunit antibody
    • Hydroxyacyl Coenzyme A (CoA) dehydrogenase beta subunit antibody
    • Hydroxyacyl Coenzyme A dehydrogenase antibody
    • Hydroxyacyl Coenzyme A dehydrogenase beta subunit antibody
    • Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) beta subunit antibody
    • MGC87480 antibody
    • Mitochondrial trifunctional enzyme beta subunit antibody
    • Mitochondrial trifunctional protein beta subunit antibody
    • MSTP 029 antibody
    • MSTP029 antibody
    • MTPB antibody
    • TP beta antibody
    • TP-beta antibody
    • TPbeta antibody
    • Trifunctional enzyme subunit beta antibody
    • Trifunctional enzyme subunit beta mitochondrial antibody
    • Trifunctional protein antibody
    see all

Images

  • All lanes : Anti-HADHB antibody (ab230667) at 1/500 dilution

    Lane 1 : BT-474 (Human ductal breast epithelial tumor cell line) cell extracts
    Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell extracts
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell extracts
    Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts
    Lane 6 : Mouse liver extracts
    Lane 7 : Mouse kidney extracts
    Lane 8 : Mouse heart extracts
    Lane 9 : Rat liver extracts

    Developed using the ECL technique.

    Predicted band size: 51 kDa

  • A549 (Human lung carcinoma cell line) cells stained for HADHB using ab230667 at a dilution of 1/10 in ICC/IF.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human heart tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreas tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.

References

ab230667 has not yet been referenced specifically in any publications.

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