Key features and details
- Rabbit polyclonal to HADHB
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-HADHB antibody
See all HADHB primary antibodies
DescriptionRabbit polyclonal to HADHB
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Mouse HADHB aa 1-259. (Expressed in E.coli).
MTTILTSTFRNLSTTSKWALRSSIRPLSCSSQLHSAPAVQTKSKKTLAKP NMKNIVVVEGVRIPFLLSGTSYKDLMPHDLARAALSGLLHRTNIPKDVVD YIIFGTVIQEVKTSNVAREAALGAGFSDKTPAHTVTMACISSNQAMTTAV GLIASGQCDVVVAGGVELMSDVPIRHSRNMRKMMLDLNKAKTLGQRLSLL SKFRLNFLSPELPAVAEFSTNETMGHSADRLAAAFAVSRMEQDEYALRSH SLAKKAQDE
Database link: Q99JY0
- WB: Mouse testis, lung and heart lysate; Recombinant mouse HADHB protein. IHC-P: Mouse brain tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 51 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
PathwayLipid metabolism; fatty acid beta-oxidation.
Involvement in diseaseDefects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
Sequence similaritiesBelongs to the thiolase family.
- Information by UniProt
- 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
- 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
- 3 ketoacyl Coenzyme A thiolase antibody
Formalin-fixed, paraffin-embedded mouse brain tissue stained for HADHB using ab233447 at 20 µg/mL in immunohistochemical analysis. DAB staining.
All lanes : Anti-HADHB antibody (ab233447) at 1 µg/ml
Lane 1 : Mouse lung lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse testis lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 51 kDa
Anti-HADHB antibody (ab233447) at 2 µg/ml + Recombinant mouse HADHB protein
Predicted band size: 51 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233447 has not yet been referenced specifically in any publications.