Overview

  • Product name
    Anti-HAI-1 antibody [EPR15652]
  • Description
    Rabbit monoclonal [EPR15652] to HAI-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HAI-1 aa 150-250. The exact sequence is proprietary.
    Database link: O43278

  • Positive control
    • Human placenta, HCT116 and HeLa cell lysates; Human transitional cell bladder carcinoma tissue.
  • General notes

     

     

    Protein previously labeled as HGFA Inhibitor 1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15652
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).
  • Sequence similarities
    Contains 2 BPTI/Kunitz inhibitor domains.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MANSC domain.
  • Domain
    This inhibitor contains two inhibitory domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAI 1 antibody
    • HAI-1 antibody
    • HAI1 antibody
    • Hepatocyte growth factor activator inhibitor type 1 antibody
    • Kunitz-type protease inhibitor 1 antibody
    • SPINT1 antibody
    • SPIT1_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human transitional cell carcinoma of bladder sections labeling HAI-1 with ab189511 at a 1/100 dilution. Secondary antibody used was a ready to use HRP polymer for rabbit IgG. Counterstained with hematoxylin.

  • Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 58 kDa

  • Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/50000 dilution + HCT116 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 58 kDa

  • Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/50000 dilution + Human placenta lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 58 kDa

References

This product has been referenced in:
  • Sun P  et al. Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells. Oncotarget 9:12682-12694 (2018). Read more (PubMed: 29560101) »
  • Zhu L  et al. Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene 35:5388-5399 (2016). IHC-P ; Human . Read more (PubMed: 27109105) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH9,0
Permeabilization
Yes - Wash - Buffer from Dako with Tween
Specification
Stomach
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Oct 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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