Product nameAnti-hair cortex Cytokeratin / K40 antibody [AE13]
See all hair cortex Cytokeratin / K40 primary antibodies
DescriptionMouse monoclonal [AE13] to hair cortex Cytokeratin / K40
SpecificityThis antibody is specific for the family of hair cortex keratins.
Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Full length protein corresponding to Human hair cortex Cytokeratin/ K40.
This antibody is an excellent marker for hair and nail differentiation.
Previously labelled as hair cortex Cytokeratin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Primary antibody notesThis antibody is an excellent marker for hair and nail differentiation.
Our Abpromise guarantee covers the use of ab16113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||1/20 - 1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMay play a role in late hair differentiation.
Tissue specificityExpressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level).
Sequence similaritiesBelongs to the intermediate filament family.
Developmental stageDuring differentiation of the hair, it is one of the last keratins expressed.
- Information by UniProt
- CK-40 antibody
- Cytokeratin-40 antibody
- hair cortex Cytokeratin antibody
ab16113 at 1/100 staining mouse skin tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antogen retrieval step was performed. The tissue was incubated with the antibody for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows hair cortex cytokeratin / K40 staining in green with DAPI nuclei counterstain in blue.
ab16113 staining hair cortex Cytokeratin / K40 in adult mouse nail by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/200 dilution, for 12 hours at 4°C. A Biotin-conjugated goat anti-mouse monoclonal was used as secondary antibody at a 1/400 dilution.
All lanes : Anti-hair cortex Cytokeratin / K40 antibody [AE13] (ab16113) at 1 µg/ml
Lane 1 :
Mouse skin tissue lysate (14 days) (ab7271)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 - 46 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
This product has been referenced in:
- Avigad Laron E et al. The Mesenchymal Niche of the Hair Follicle Induces Regeneration by Releasing Primed Progenitors from Inhibitory Effects of Quiescent Stem Cells. Cell Rep 24:909-921.e3 (2018). Read more (PubMed: 30044987) »
- Leng X et al. Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay". Exp Dermatol N/A:N/A (2018). Read more (PubMed: 30033549) »