Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)
Key features and details
- Mouse monoclonal [AE13] to hair cortex Cytokeratin/K40
- Suitable for: IHC-Fr, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-hair cortex Cytokeratin/K40 antibody [AE13]
See all hair cortex Cytokeratin/K40 primary antibodies -
Description
Mouse monoclonal [AE13] to hair cortex Cytokeratin/K40 -
Host species
Mouse -
Specificity
This antibody is specific for the family of hair cortex keratins. -
Tested applications
Suitable for: IHC-Fr, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length native protein (purified) corresponding to Human hair cortex Cytokeratin/K40.
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General notes
This antibody is an excellent marker for hair and nail differentiation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody is an excellent marker for hair and nail differentiation. -
Clonality
Monoclonal -
Clone number
AE13 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (4) |
Use at an assay dependent concentration.
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IHC-P | (3) |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa).
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|
Flow Cyt |
1/20 - 1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa). |
Flow Cyt
1/20 - 1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play a role in late hair differentiation. -
Tissue specificity
Expressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level). -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
During differentiation of the hair, it is one of the last keratins expressed. - Information by UniProt
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Database links
- Entrez Gene: 125115 Human
- Entrez Gene: 406221 Mouse
- SwissProt: Q6A162 Human
- SwissProt: Q6IFX3 Mouse
- Unigene: 534929 Human
- Unigene: 422372 Mouse
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Alternative names
- CK-40 antibody
- Cytokeratin-40 antibody
- hair cortex Cytokeratin antibody
see all
Images
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All lanes : Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113) at 1 µg/ml
Lane 1 :Mouse skin tissue lysate (14 days) (ab7271)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 - 46 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab16113 has been referenced in 36 publications.
- Joseph CB et al. Meta-GWAS Reveals Novel Genetic Variants Associated with Urinary Excretion of Uromodulin. J Am Soc Nephrol 33:511-529 (2022). PubMed: 35228297
- Polkoff KM et al. LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis. Sci Rep 12:9104 (2022). PubMed: 35650234
- Xie S et al. Self-assembled complete hair follicle organoids by coculture of neonatal mouse epidermal cells and dermal cells in Matrigel. Ann Transl Med 10:767 (2022). PubMed: 35965801
- Jung SY et al. Wnt-activating human skin organoid model of atopic dermatitis induced by Staphylococcus aureus and its protective effects by Cutibacterium acnes. iScience 25:105150 (2022). PubMed: 36193049
- Oka A et al. Alopecia areata susceptibility variant in MHC region impacts expressions of genes contributing to hair keratinization and is involved in hair loss. EBioMedicine 57:102810 (2020). PubMed: 32580135