Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

    Key features and details

    • Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgM

    Overview

    • Product name

      Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted
      See all Hairy Cell Leukemia primary antibodies

    • Description

      Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted

    • Host species

      Mouse

    • Specificity

      This antibody reacts with over 97% of hairy cell leukaemias as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      BALB/C mice were injected with the Deau cell line established from a large cell lymphoma.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.

    • Storage buffer

      pH: 7.3
      Preservative: 0.05% Sodium azide

      Inert stabilizer
    • Concentration information loading...

    • Clonality

      Monoclonal

    • Clone number

      DBA.44

    • Myeloma

      unknown

    • Isotype

      IgM

    • Light chain type

      kappa

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab857 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration.
    Notes
    IHC-P
    Use at an assay dependent concentration.

    Target

    • Cellular localization

      Nuclear

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

      Immunohistochemistry (Formalin fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Hairy Cell Leukemia with ab857.

    References (0)

    Publishing research using ab857? Please let us know so that we can cite the reference in this datasheet.

    ab857 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    BATCH NUMBER 143106 ORDER NUMBER 105179 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species PRIMARY ANTIBODY DBA.44 for 1hr at RT DETECTION METHOD DAB+ POSITIVE AND NEGATIVE CONTROLS USED Spleen with Hairy cell leukemia ANTIBODY STORAGE CONDITIONS Antibody was received 7 days after shipment due to US customs hold. Antibody was received hot! FIXATION OF SAMPLE Formalin ANTIGEN RETRIEVAL Enzymatic using DAKO proteolytic enzyme for 5 minutes at room temperature. BLOCKING CONDITIONS H2O2 SECONDARY ANTIBODY DAKO LSAB2 HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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    Answer

    Thank you for contacting us for technical support, I'm very sorry to hear you are experiencing problems with ab857 and it does appear that the delayed shipment of the antibody damaged it as your protocol looks very good (you still may need to increase the incubation time to overnight depending on the tissue type). I can arrange a replacement vial or offer you a refund if you would prefer, could you please let me know what you prefer and I will arrange this immediately? I look forward to hearing from you,

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