Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

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Overview

  • Product name
    Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted
    See all Hairy Cell Leukemia primary antibodies
  • Description
    Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted
  • Host species
    Mouse
  • Specificity
    This antibody reacts with over 97% of hairy cell leukaemias as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were injected with the Deau cell line established from a large cell lymphoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    DBA.44
  • Myeloma
    unknown
  • Isotype
    IgM
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.

Target

  • Cellular localization
    Nuclear

References

ab857 has not yet been referenced specifically in any publications.

Publishing research using ab857? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

BATCH NUMBER 143106 ORDER NUMBER 105179 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species PRIMARY ANTIBODY DBA.44 for 1hr at RT DETECTION METHOD DAB+ POSITIVE AND NEGATIVE CONTROLS USED Spleen with Hairy cell leukemia ANTIBODY STORAGE CONDITIONS Antibody was received 7 days after shipment due to US customs hold. Antibody was received hot! FIXATION OF SAMPLE Formalin ANTIGEN RETRIEVAL Enzymatic using DAKO proteolytic enzyme for 5 minutes at room temperature. BLOCKING CONDITIONS H2O2 SECONDARY ANTIBODY DAKO LSAB2 HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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Answer

Thank you for contacting us for technical support, I'm very sorry to hear you are experiencing problems with ab857 and it does appear that the delayed shipment of the antibody damaged it as your protocol looks very good (you still may need to increase the incubation time to overnight depending on the tissue type). I can arrange a replacement vial or offer you a refund if you would prefer, could you please let me know what you prefer and I will arrange this immediately? I look forward to hearing from you,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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