Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (ab857)

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Key features and details

  • Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted
    See all Hairy Cell Leukemia primary antibodies

  • Description

    Mouse monoclonal [DBA.44] to Hairy Cell Leukemia, prediluted

  • Host species

    Mouse

  • Specificity

    This antibody reacts with over 97% of hairy cell leukaemias as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    BALB/C mice were injected with the Deau cell line established from a large cell lymphoma.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    DBA.44

  • Myeloma

    unknown

  • Isotype

    IgM

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.

Target

  • Cellular localization

    Nuclear

References (0)

Publishing research using ab857? Please let us know so that we can cite the reference in this datasheet.

ab857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

BATCH NUMBER 143106 ORDER NUMBER 105179 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species PRIMARY ANTIBODY DBA.44 for 1hr at RT DETECTION METHOD DAB+ POSITIVE AND NEGATIVE CONTROLS USED Spleen with Hairy cell leukemia ANTIBODY STORAGE CONDITIONS Antibody was received 7 days after shipment due to US customs hold. Antibody was received hot! FIXATION OF SAMPLE Formalin ANTIGEN RETRIEVAL Enzymatic using DAKO proteolytic enzyme for 5 minutes at room temperature. BLOCKING CONDITIONS H2O2 SECONDARY ANTIBODY DAKO LSAB2 HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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Answer

Thank you for contacting us for technical support, I'm very sorry to hear you are experiencing problems with ab857 and it does appear that the delayed shipment of the antibody damaged it as your protocol looks very good (you still may need to increase the incubation time to overnight depending on the tissue type). I can arrange a replacement vial or offer you a refund if you would prefer, could you please let me know what you prefer and I will arrange this immediately? I look forward to hearing from you,

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