Key features and details
- Mouse monoclonal [OTI3A2] to Hamartin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Hamartin antibody [OTI3A2]
See all Hamartin primary antibodies
DescriptionMouse monoclonal [OTI3A2] to Hamartin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Hamartin aa 836-1164. Produced in E.coli.
SNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYR KELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKL QARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEA AEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGG GGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSK SFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNL DGPHPSPPTPDSVGQLHIMDYNETHHEHS
Database link: NP_000359
- HEK293T cell lysate transfected with pCMV6-ENTRY Hamartin cDNA; Human breast, colon, colon adenocarcinoma, pancreas, endometrium adenocarcinoma, prostate and prostate carcinoma tissues.
The clone number has been updated from 3A2 to OTI3A2, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 10th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesab170164 is purified from TCS by affinity chromatography.
Our Abpromise guarantee covers the use of ab170164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 130 kDa.|
FunctionIn complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
Tissue specificityHighly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Involvement in diseaseDefects in TSC1 are the cause of tuberous sclerosis type 1 (TSC1) [MIM:191100]. It is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TS1C is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC) [MIM:607341]. FCDBC is a subtype of cortical displasias linked to chronic intractable epilepsy. Cortical dysplasias display a broad spectrum of structural changes, which appear to result from changes in proliferation, migration, differentiation, and apoptosis of neuronal precursors and neurons during cortical development.
DomainThe C-terminal putative coiled-coil domain is necessary for interaction with TSC2.
modificationsPhosphorylation at Ser-505 does not affect interaction with TSC2. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Membrane. At steady state found in association with membranes.
- Information by UniProt
- Hamartin antibody
- kiaa0243 antibody
- LAM antibody
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human adult heart tissue tissue labeling Hamartin with ab170164 at 1/150 dilution.
All lanes : Anti-Hamartin antibody [OTI3A2] (ab170164) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hamartin cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 130 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218987)
ab170164 has not yet been referenced specifically in any publications.