Overview

  • Product name

    Hamster IgG ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 3 3.5%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.7%
  • Sample type

    Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.08 ng/ml
  • Range

    0.31 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Fetal Bovine Serum 97 94.5% - 99%
    serum free media 92 87.3% - 94.4%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Syrian hamster
    Does not react with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Cow, Dog, Human, Armenian hamster
  • Product overview

    Abcam’s IgG Hamster in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Syrian Hamster IgG protein in serum samples.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    There are three classes of immunoglobulins in Hamster: IgA, IgM, and IgG. IgG is the most abundant immunoglobulin in serum and is equally distributed in blood and tissue. In Hamster, the IgG class is further divided into two subclasses: IgG1, IgG2; however some research suggests that a third subclass, IgG3, is also found in outbred strains, and only some inbred strains of Hamster serum. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y-like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.


    IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically hamster serum and plasma samples contain about 7 to 10 mg/ml of IgG.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Hamster IgG Capture Antibody 1 x 600µl
    10X Hamster IgG Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BC 1 x 6ml
    Hamster IgG Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

Applications

Our Abpromise guarantee covers the use of ab200010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Linearity of IgG hamster by testing hamster Serum assayed at a dilution of 1:2 Million (undiluted), and interpolating the undiluted and diluted amounts and multiplying by the dilution factors (D.F.) is plotted.

Protocols

References

ab200010 has not yet been referenced specifically in any publications.

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