Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.08 ng/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Hamster, Syrian hamster
Product nameHamster IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Serum 3 3.5% Inter-assay Sample n Mean SD CV% Serum 3 6.7%
Assay typeSandwich (quantitative)
Range0.31 ng/ml - 20 ng/ml
Sample specific recovery Sample type Average % Range Fetal Bovine Serum 97 94.5% - 99% serum free media 92 87.3% - 94.4%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Hamster, Syrian hamster
Does not react with: Sheep, Goat, Cow, Armenian hamster
Hamster IgG ELISA Kit (ab200010) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Hamster IgG with 0.08 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
There are three classes of immunoglobulins in Hamster: IgA, IgM, and IgG. IgG is the most abundant immunoglobulin in serum and is equally distributed in blood and tissue. In Hamster, the IgG class is further divided into two subclasses: IgG1, IgG2; however some research suggests that a third subclass, IgG3, is also found in outbred strains, and only some inbred strains of Hamster serum. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y-like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically hamster serum and plasma samples contain about 7 to 10 mg/ml of IgG.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Hamster IgG Capture Antibody 1 x 600µl 10X Hamster IgG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BC 1 x 6ml Hamster IgG Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Background-subtracted data values (mean +/- SD) are graphed.
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Linearity of IgG hamster by testing hamster Serum assayed at a dilution of 1:2 Million (undiluted), and interpolating the undiluted and diluted amounts and multiplying by the dilution factors (D.F.) is plotted.
ab200010 has not yet been referenced specifically in any publications.