Anti-HAND1 antibody (ab196622)
Key features and details
- Rabbit polyclonal to HAND1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HAND1 antibody
See all HAND1 primary antibodies -
Description
Rabbit polyclonal to HAND1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human HAND1 (internal sequence).
Database link: O96004 -
Positive control
- A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/500.
|
Notes |
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ICC/IF
1/100 - 1/500. |
Target
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Function
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box). -
Tissue specificity
Heart. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity. - Information by UniProt
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Database links
- Entrez Gene: 9421 Human
- Entrez Gene: 15110 Mouse
- Entrez Gene: 59112 Rat
- Omim: 602406 Human
- SwissProt: O96004 Human
- SwissProt: Q64279 Mouse
- SwissProt: P97832 Rat
- Unigene: 152531 Human
see all -
Alternative names
- autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
- Basic helix loop helix transcription factor HAND1 antibody
- bHLHa27 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab196622 has been referenced in 3 publications.
- Lewis-Israeli YR et al. Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells. J Vis Exp N/A:N/A (2021). PubMed: 34605811
- Musah S et al. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip. Nat Protoc 13:1662-1685 (2018). PubMed: 29995874
- Musah S et al. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip. Nat Biomed Eng 1:N/A (2017). PubMed: 29038743