Overview

  • Product name

  • Description

    Rabbit polyclonal to HAND1
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous levels of total HAND1 protein.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HAND1 (internal sequence).
    Database link: O96004

  • Positive control

    • A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab196622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.

Target

  • Function

    Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
  • Tissue specificity

    Heart.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
    • Basic helix loop helix transcription factor HAND1 antibody
    • bHLHa27 antibody
    • Class A basic helix-loop-helix protein 27 antibody
    • eHAND antibody
    • Extraembryonic tissues antibody
    • Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1 antibody
    • HAND 1 antibody
    • HAND1 antibody
    • HAND1_HUMAN antibody
    • Heart and neural crest derivatives expressed 1 antibody
    • Heart and neural crest derivatives expressed protein 1 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 1 antibody
    • Hxt antibody
    • Thing 1 antibody
    • Thing1 antibody
    • Thing1 antibody
    see all

Images

  • Immunofluorscence analysis of A459 cells labeling HAND1 with ab196622 at a 1/100 dilution. Right image treated with synthesized peptide.

References

This product has been referenced in:

  • Musah S  et al. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip. Nat Biomed Eng 1:N/A (2017). Read more (PubMed: 29038743) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse embryonic heart, day E10.5)
Permeabilization
No
Specification
Mouse embryonic heart, day E10.5
Blocking step
PBS with Saponin as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 03 2017

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