Overview

  • Product name
  • Description
    Rabbit polyclonal to HAND1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human HAND1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab47013.)

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Brain, Heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab46822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25.8 kDa (predicted molecular weight: 25.8 kDa).

Target

  • Function
    Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
  • Tissue specificity
    Heart.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
    • Basic helix loop helix transcription factor HAND1 antibody
    • bHLHa27 antibody
    • Class A basic helix-loop-helix protein 27 antibody
    • eHAND antibody
    • Extraembryonic tissues antibody
    • Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1 antibody
    • HAND 1 antibody
    • HAND1 antibody
    • HAND1_HUMAN antibody
    • Heart and neural crest derivatives expressed 1 antibody
    • Heart and neural crest derivatives expressed protein 1 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 1 antibody
    • Hxt antibody
    • Thing 1 antibody
    • Thing1 antibody
    • Thing1 antibody
    see all

Images

  • All lanes : Anti-HAND1 antibody (ab46822) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 25.8 kDa
    Observed band size: 25.8 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Yang C  et al. Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis. Hum Mol Genet 26:3031-3045 (2017). Read more (PubMed: 28521042) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (293T)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
60 µg
Treatment
transfected with pCMV-HAND1
Specification
293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 18 2015

Answer

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

I have conducted a search for you and found the following antibodies:

FOXJ2 - 2 antibodies but not tested yet in Rat (suitable for Western blot)

ab22857, ab49160

HAND1 - 4 antibodies but not tested yet in Rat (suitable for Western blot)

ab46822, ab52767, ab86009, ab104320



NFAT5

ab3446 - reacts with Rat

ab105886 - predicted to react with Rat





Nkx2-3

ab49162 - predicted to react with Rat



ab66366- predicted to react with Rat





Nkx2-4

ab48331 - predicted to react with Rat



ab49162 - predicted to react with Rat



ab66366 - predicted to react with Rat


If you have specific questions about the products identified during the search, please do not hesitate to contact me.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Nov 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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