Recombinant
RabMAb

Recombinant Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

Submit a review Submit a question

Overview

  • Product name

    Anti-HAND2 antibody [EPR19451] - BSA and Azide free
    See all HAND2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19451] to HAND2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse HAND2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q61039

  • General notes

    Ab240353 is the carrier-free version of ab200040. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240353 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.

  • Tissue specificity

    Heart.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • Developmental stage

    Expressed in the fetal heart.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AI225906 antibody
    • AI661148 antibody
    • autonomic nervous system and neural crest derivatives-expressed protein 2 antibody
    • Basic helix loop helix transcription factor HAND2 antibody
    • bHLHa26 antibody
    • Class A basic helix-loop-helix protein 26 antibody
    • Deciduum antibody
    • Deciduum heart autonomic nervous system and neural crest derivatives expressed protein 2 antibody
    • DHAND 2 antibody
    • dHAND antibody
    • DHAND2 antibody
    • Ehand 2 antibody
    • Ehand2 antibody
    • FLJ16260 antibody
    • HAND 2 antibody
    • Hand2 antibody
    • HAND2_HUMAN antibody
    • Heart and neural crest derivatives expressed 2 antibody
    • Heart and neural crest derivatives expressed protein 2 antibody
    • heart and neural crest derivatives expressed transcript 2 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 2 antibody
    • Hed antibody
    • Highly similar to dHAND M musculus antibody
    • MGC125303 antibody
    • MGC125304 antibody
    • Th 2 antibody
    • Th2 antibody
    • Thing 2 antibody
    • Thing2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling HAND2 with ab200040 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in multiple tissues of E14.5 mouse embryo (PMID: 25248475). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 embryonic tissue labeling HAND2 with ab200040 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in multiple rat embryonic (E14.5) tissues (PMID: 25248475). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryonic adrenal gland tissue labeling HAND2 with ab200040 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in mouse embryonic (E14.5) ganglion and adrenal gland (PMID: 25248475). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 embryonic gut tissue labeling HAND2 with ab200040 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in rat E14.5 embryonic gut (PMID: 25248475). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunocytochemistry/ Immunofluorescence - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling HAND2 with ab200040 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in Neuro-2a cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

  • Immunocytochemistry/ Immunofluorescence - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunocytochemistry/ Immunofluorescence - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling HAND2 with ab200040 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in SH-SY5Y cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

  • Flow Cytometry - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Flow Cytometry - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (mouse neuroblastoma cell line) cell line labeling HAND2 with ab200040 at 1/400 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

  • Immunoprecipitation - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)
    Immunoprecipitation - Anti-HAND2 antibody [EPR19451] - BSA and Azide free (ab240353)

    HAND2 was immunoprecipitated from 0.35 mg of SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate with ab200040 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200040 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: SH-SY5Y whole cell lysate 10 µg (Input). 

    Lane 2: ab200040 IP in SH-SY5Y whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200040 in SH-SY5Y whole cell lysate.

    Exposure time: Less than 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200040).

References

ab240353 has not yet been referenced specifically in any publications.

Publishing research using ab240353? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab240353.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com