The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000.
1/1000 - 1/5000.
The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.
Western blot - Anti-Hantavirus glycoprotein G2 antibody [10B8] (ab34763)
ab34763 at 1 µg/mL used in Western blot of recombinant full-length G2 glycoprotein of Kazan hantavirus at 500 ng per lane.
Zvirbliene A et al. The use of chimeric virus-like particles harbouring a segment of hantavirus Gc glycoprotein to generate a broadly-reactive hantavirus-specific monoclonal antibody. Viruses6:640-60 (2014).
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