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    hantavirus-igg-elisa-kit-ab247195.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Other Viruses
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Hantavirus IgG ELISA kit (ab247195)

  • Datasheet
  • SDS
  • Protocol Booklet
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Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (qualitative)
    • Reacts with: Human

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    Overview

    • Product name

      Hantavirus IgG ELISA kit
      See all Hantavirus kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      sample 24 0.45nM 3.61%
      sample 24 1.333nM 6.41%
      sample 24 1.264nM 4.78%
      Inter-assay
      Sample n Mean SD CV%
      sample 12 0.45nM 3.61%
      sample 12 1.333nM 6.41%
      sample 12 1.264nM 4.78%
    • Sample type

      Serum, Hep Plasma, Cit plasma
    • Assay type

      Sandwich (qualitative)
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Hantavirus IgG ELISA Kit (ab247195) is designed for the qualitative determination of IgG class antibodies against Hantavirus in human serum or plasma (citrate, heparin). 


      The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.

    • Notes

      Hantaviruses are negative sense RNA viruses in the Bunyaviridae family. Humans may be infected with Hantaviruses through urine, saliva or contact with rodent waste products. Some Hantaviruses may lead to serious diseases in humans, such as hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Human infections of Hantaviruses have almost entirely been linked to human contact with rodent excrement, but recent human to human transmission has been reported with the Andes virus in South America. Hantavirus has an incubation time of two to four weeks in humans before symptoms of infection occur. The symptoms of HFRS can be split into five phases:

      • Febrile phase: Symptoms include fever, chills, sweaty palms, diarrhea, malaise, headaches, nausea, abdominal and back pain, respiratory problems such as the ones common in influenza virus infection, as well as gastro-intestinal problems. These symptoms normally occur for three to seven days and arise about two to three weeks after exposure.
      • Hypotensive phase: This occurs when the blood platelet levels drop and symptoms can lead to tachycardia and hypoxemia. This phase can last for 2 days.
      • Oliguric phase: This phase lasts for three to seven days and is characterized by the onset of renal failure and proteinuria occurs.
      • Diuretic phase: This is characterized by diuresis of three to six liters per day, which can last for a couple of days up to weeks.
      • Convalescent phase: This is normally when recovery occurs and symptoms begin to improve.

      Regions especially affected by HFRS include China, the Korean Peninsula, Russia (Hantaan, Puumala and Seoul viruses), and northern and western Europe (Puumala and Dobrava virus).

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      20X Washing Solution White cap 1 x 50ml
      anti-human IgG HRP conjugate 1 x 20ml
      Cover Foil 1 unit
      Hantavirus Coated Microplate (IgG) 1 unit
      IgG Cut-off Control 1 x 3ml
      IgG Negative Control 1 x 2ml
      IgG Positive Control 1 x 2ml
      IgG Sample Diluent 1 x 100ml
      Stop Solution red cap 1 x 15ml
      TMB Substrate Solution Yellow cap 1 x 15ml
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Other Viruses
    • Relevance

      Hantavirus is a negative sense single stranded RNA virus of the family Bunyaviridae. Hantaviruses normally infect rodents such as mice and voles. In humans the virus causes two main patterns of disease: a bleeding disorder with low blood pressure and kidney failure (haemorrhagic fever with renal syndrome); and an illness with lung involvement and breathlessness, progressing to shock with high mortality (Hantavirus pulmonary syndrome). There are a number of strains of Hantavirus which vary in their distribution throughout the world, and also vary in terms of the severity of illness that they produce.
    • Alternative names

      • Hanta virus
      • Hantaan virus

    Images

    • Serologic ELISA assay principle
      Serologic ELISA assay principle
      Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

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