Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Hantavirus nucleocapsid protein antibody [5E11]
See all Hantavirus nucleocapsid protein primary antibodies -
DescriptionMouse monoclonal [5E11] to Hantavirus nucleocapsid protein
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Host speciesMouse
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Tested applicationsSuitable for: Indirect ELISA, WB, ICC/IFmore details
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Species reactivityReacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1 Not yet tested in other species.
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Immunogen
Recombinant fragment corresponding to Hantavirus nucleocapsid protein.
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EpitopeThe epitope recognized by this antibody is localized at N-terminus, between amino acids 1 and 45 of the hantavirus nucleocapsid protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR148555-9 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number5E11
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab34757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | 1/1000 - 1/10000. | |
| WB | 1/1000 - 1/5000. | |
| ICC/IF | Use at an assay dependent dilution. PubMed: 18687679 |
Target
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RelevanceThe members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.
Images
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![Western blot - Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)](https://www.abcam.com/ps/products/34/ab34757/Images/ab34757_1.jpg)
Western blot - Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)ab34757 at 1 µg/mL used in Western blot of recombinant nucleocapsid proteins.
Lane 1 Sotkamo NP
Lanes 2 & 3 Vranica NP
Lane 4 Kazan NP
References
This product has been referenced in:
- Mertens M et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes 43:177-91 (2011). Read more (PubMed: 21598005) »
- Wang Y et al. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem 283:28297-304 (2008). ICC/IF . Read more (PubMed: 18687679) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"