Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)
![Western blot - Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)](https://www.abcam.com/ps/products/34/ab34757/Images/ab34757-47973-anti-hantavirus-nucleocapsid-protein-antibody-5e11-western-blot.jpg "Western blot - Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)")
Key features and details
- Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein
- Suitable for: Indirect ELISA, WB, ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-Hantavirus nucleocapsid protein antibody [5E11]
See all Hantavirus nucleocapsid protein primary antibodies -
Description
Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein -
Host species
Mouse -
Tested applications
Suitable for: Indirect ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant fragment corresponding to Hantavirus nucleocapsid protein.
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Epitope
The epitope recognized by this antibody is localized at N-terminus, between amino acids 1 and 45 of the hantavirus nucleocapsid protein. -
General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR148555-9 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5E11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA |
1/1000 - 1/10000.
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| WB |
1/1000 - 1/5000.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 18687679
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| Notes |
|---|
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Indirect ELISA
1/1000 - 1/10000. |
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WB
1/1000 - 1/5000. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 18687679 |
Target
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Relevance
The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease. -
Database links
- Entrez Gene: 3293257 Other species
- SwissProt: P05133 Other species
Images
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Western blot - Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)
ab34757 at 1 µg/mL used in Western blot of recombinant nucleocapsid proteins.
Lane 1 Sotkamo NP
Lanes 2 & 3 Vranica NP
Lane 4 Kazan NP
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab34757 has been referenced in 5 publications.
- Koban R et al. Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection. Viruses 12:N/A (2020). PubMed: 33198291
- Bourquain D et al. Puumala and Tula Virus Differ in Replication Kinetics and Innate Immune Stimulation in Human Endothelial Cells and Macrophages. Viruses 11:N/A (2019). PubMed: 31540120
- Sabino-Santos G et al. Natural infection of Neotropical bats with hantavirus in Brazil. Sci Rep 8:9018 (2018). PubMed: 29899544
- Mertens M et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes 43:177-91 (2011). PubMed: 21598005
- Wang Y et al. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem 283:28297-304 (2008). ICC/IF . PubMed: 18687679