Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34759)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Hantavirus nucleocapsid protein antibody [5E11]
See all Hantavirus nucleocapsid protein primary antibodies -
DescriptionMouse monoclonal [5E11] to Hantavirus nucleocapsid protein
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Host speciesMouse
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SpecificityThis antibody reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It cross-reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1, nor with nucleocapsid proteins of measles and mumps viruses.
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Tested applicationsSuitable for: ICC/IFmore details
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Species reactivityReacts with hantavirus. Not yet tested in other species.
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Immunogen
Recombinant fragment -recombinant chimeric protein harbouring N-terminal segment (1-120 aa) of Puumala hantavirus (strain Vranica-Hällnäs) nucleocapsid protein, expressed in yeast S.cerevisiae.
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EpitopeThe epitope recognized by this antibody is localized at the N-terminus, between amino acids 1 and 45 of hantavirus nucleocapsid protein.
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General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone number5E11
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab34759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1 - 1/20. |
Target
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RelevanceThe members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.
References
This product has been referenced in:
- Perelygina L et al. Persistent infection of human fetal endothelial cells with rubella virus. PLoS One 8:e73014 (2013). WB ; Human . Read more (PubMed: 23940821) »
- Schlegel M et al. Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirus. Arch Virol 157:2179-87 (2012). Read more (PubMed: 22828778) »
