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  1. Link

    hantavirus-nucleocapsid-protein-antibody-5e11-ab34759.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Other Viruses
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Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34759)

  • Datasheet
  • SDS
Submit a review Submit a question References (4)

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Key features and details

  • Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein
  • Suitable for: ICC/IF
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Hantavirus nucleocapsid protein antibody [5E11]
    See all Hantavirus nucleocapsid protein primary antibodies
  • Description

    Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein
  • Host species

    Mouse
  • Specificity

    This antibody reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It cross-reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1, nor with nucleocapsid proteins of measles and mumps viruses.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant fragment -recombinant chimeric protein harbouring N-terminal segment (1-120 aa) of Puumala hantavirus (strain Vranica-Hällnäs) nucleocapsid protein, expressed in yeast S.cerevisiae.

  • Epitope

    The epitope recognized by this antibody is localized at the N-terminus, between amino acids 1 and 45 of hantavirus nucleocapsid protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituent: Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    5E11
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • single stranded RNA negative strand virus
    • Other Viruses

Associated products

  • Alternative Versions

    • Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab34759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1 - 1/20.
Notes
ICC/IF
1/1 - 1/20.

Target

  • Relevance

    The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.
  • Database links

    • Entrez Gene: 3293257 Other species
    • SwissProt: P05133 Other species

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab34759? Please let us know so that we can cite the reference in this datasheet.

    ab34759 has been referenced in 4 publications.

    • Perelygina L  et al. Persistent infection of human fetal endothelial cells with rubella virus. PLoS One 8:e73014 (2013). WB ; Human . PubMed: 23940821
    • Schlegel M  et al. Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirus. Arch Virol 157:2179-87 (2012). PubMed: 22828778
    • Mertens M  et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes 43:177-91 (2011). PubMed: 21598005
    • Kucinskaite-Kodze I  et al. Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples. Arch Virol 156:443-56 (2011). PubMed: 21161552

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