Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34759)
Key features and details
- Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein
- Suitable for: ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-Hantavirus nucleocapsid protein antibody [5E11]
See all Hantavirus nucleocapsid protein primary antibodies -
Description
Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein -
Host species
Mouse -
Specificity
This antibody reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It cross-reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1, nor with nucleocapsid proteins of measles and mumps viruses. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant fragment -recombinant chimeric protein harbouring N-terminal segment (1-120 aa) of Puumala hantavirus (strain Vranica-Hällnäs) nucleocapsid protein, expressed in yeast S.cerevisiae.
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Epitope
The epitope recognized by this antibody is localized at the N-terminus, between amino acids 1 and 45 of hantavirus nucleocapsid protein. -
General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5E11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab34759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1 - 1/20. |
Target
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Relevance
The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease. -
Database links
- Entrez Gene: 3293257 Other species
- SwissProt: P05133 Other species
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (4)
ab34759 has been referenced in 4 publications.
- Perelygina L et al. Persistent infection of human fetal endothelial cells with rubella virus. PLoS One 8:e73014 (2013). WB ; Human . PubMed: 23940821
- Schlegel M et al. Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirus. Arch Virol 157:2179-87 (2012). PubMed: 22828778
- Mertens M et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes 43:177-91 (2011). PubMed: 21598005
- Kucinskaite-Kodze I et al. Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples. Arch Virol 156:443-56 (2011). PubMed: 21161552
