Anti-HAO1/GOX antibody (ab224577)
Key features and details
- Rabbit polyclonal to HAO1/GOX
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HAO1/GOX antibody
See all HAO1/GOX primary antibodies -
Description
Rabbit polyclonal to HAO1/GOX -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human HAO1/GOX aa 1-100.
Database link: Q9UJM8 -
Positive control
- IHC-P: Human liver and testis tissue. WB: Human liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. -
Tissue specificity
Liver. -
Pathway
Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4. -
Sequence similarities
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Contains 1 FMN hydroxy acid dehydrogenase domain. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 54363 Human
- Omim: 605023 Human
- SwissProt: Q9UJM8 Human
- Unigene: 193640 Human
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Alternative names
- (S) 2 hydroxy acid oxidase antibody
- Glycolate oxidase antibody
- GOX antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling HAO1/GOX with ab224577 at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HAO1/GOX with ab224577 at 1/1000 dilution.
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Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling HAO1/GOX with ab224577 at 1/1000 dilution.
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Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling HAO1/GOX with ab224577 at 1/1000 dilution.
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All lanes : Anti-HAO1/GOX antibody (ab224577) at 1/500 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 41 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224577 has not yet been referenced specifically in any publications.