Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6338] to HAPLN1
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-HAPLN1 antibody [EPR6338]
See all HAPLN1 primary antibodies
DescriptionRabbit monoclonal [EPR6338] to HAPLN1
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HAPLN1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P10915
- Human spleen, MCF7 and Caco2 lysates; Human transitional cell carcinoma of bladder tissue; MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionStabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Tissue specificityWidely expressed. Weakly expressed in the brain.
Sequence similaritiesBelongs to the HAPLN family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Information by UniProt
- Cartilage link protein antibody
- cartilage linking protein 1 antibody
- Cartilage-link protein antibody
All lanes : Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution
Lane 1 : MCF7 lysate
Lane 2 : Caco2 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution
Predicted band size: 40 kDa
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).
Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling HAPLN1 with purified ab181997 at 1/2000 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution + Human spleen lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 40 kDa
ab181997 has been referenced in 1 publication.
- Tajerian M et al. The hippocampal extracellular matrix regulates pain and memory after injury. Mol Psychiatry 23:2302-2313 (2018). PubMed: 30254235