Anti-HAPLN2 antibody (ab124504)

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Key features and details

  • Rabbit polyclonal to HAPLN2
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HAPLN2 antibody

  • Description

    Rabbit polyclonal to HAPLN2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A 12 amino acid synthetic peptide from near the center of Human HAPLN2 (NP_068589).

  • Positive control

    • Human brain tissue lysate; Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab124504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • Function

    Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid.

  • Tissue specificity

    Expressed only in adult brain.

  • Sequence similarities

    Belongs to the HAPLN family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Brain link protein 1 antibody
    • Bral1 antibody
    • Hapln2 antibody
    • HPLN2_HUMAN antibody
    • Hyaluronan and proteoglycan link protein 2 antibody
    see all

Images

  • Western blot - Anti-HAPLN2 antibody (ab124504)
    Western blot - Anti-HAPLN2 antibody (ab124504)
    Lane 1 : Anti-HAPLN2 antibody (ab124504) at 1 µg/ml
    Lane 2 : Anti-HAPLN2 antibody (ab124504) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN2 antibody (ab124504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN2 antibody (ab124504)
    ab124504, at 2.5 µg/ml, staining HAPLN2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-HAPLN2 antibody (ab124504)
    Immunocytochemistry/ Immunofluorescence - Anti-HAPLN2 antibody (ab124504)
    Immunofluorescence of BRAL1 in Human Brain cells using ab124504 at 20 ug/ml.

References

Publishing research using ab124504? Please let us know so that we can cite the reference in this datasheet.

ab124504 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-HAPLN2 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Dr. Sergi Bayod

Verified customer

Submitted Nov 05 2019

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