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  1. Link

    hapln2-antibody-ab124504.pdf

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Neuroscience Cell Adhesion Proteins ECM Proteins
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Anti-HAPLN2 antibody (ab124504)

  • Datasheet
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Western blot - Anti-HAPLN2 antibody (ab124504)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN2 antibody (ab124504)
  • Immunocytochemistry/ Immunofluorescence - Anti-HAPLN2 antibody (ab124504)

Key features and details

  • Rabbit polyclonal to HAPLN2
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human HAPLN2 protein (ab163721)
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Overview

  • Product name

    Anti-HAPLN2 antibody
  • Description

    Rabbit polyclonal to HAPLN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A 12 amino acid synthetic peptide from near the center of Human HAPLN2 (NP_068589).

  • Positive control

    • Human brain tissue lysate; Human brain tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human HAPLN2 protein (ab163721)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2.5 µg/ml.
WB (1)
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 37 kDa.
Notes
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 2.5 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • Function

    Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid.
  • Tissue specificity

    Expressed only in adult brain.
  • Sequence similarities

    Belongs to the HAPLN family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q9GZV7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 60484 Human
    • SwissProt: Q9GZV7 Human
    • Unigene: 410719 Human
    • Alternative names

      • Brain link protein 1 antibody
      • Bral1 antibody
      • Hapln2 antibody
      • HPLN2_HUMAN antibody
      • Hyaluronan and proteoglycan link protein 2 antibody
      see all

    Images

    • Western blot - Anti-HAPLN2 antibody (ab124504)
      Western blot - Anti-HAPLN2 antibody (ab124504)
      Lane 1 : Anti-HAPLN2 antibody (ab124504) at 1 µg/ml
      Lane 2 : Anti-HAPLN2 antibody (ab124504) at 2 µg/ml

      All lanes : Human brain tissue lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 37 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN2 antibody (ab124504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN2 antibody (ab124504)
      ab124504, at 2.5 µg/ml, staining HAPLN2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-HAPLN2 antibody (ab124504)
      Immunocytochemistry/ Immunofluorescence - Anti-HAPLN2 antibody (ab124504)
      Immunofluorescence of BRAL1 in Human Brain cells using ab124504 at 20 ug/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124504? Please let us know so that we can cite the reference in this datasheet.

    ab124504 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-HAPLN2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Hippocampus)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Specification
    Hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sergi Bayod

    Verified customer

    Submitted Nov 05 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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