Anti-Haptoglobin antibody (ab231000)
Key features and details
- Rabbit polyclonal to Haptoglobin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Haptoglobin antibody
See all Haptoglobin primary antibodies -
Description
Rabbit polyclonal to Haptoglobin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Rat Haptoglobin aa 100-350. His-tag and T7 tag. Expressed in E. coli.
Database link: P06866 -
Positive control
- WB: Recombinant rat Haptoglobin protein; mouse liver lysate; rat serum; HepG2 cell lysate. IHC-P: Rat testis stomach, liver and spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 37 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 37 kDa. |
Target
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Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3240 Human
- Entrez Gene: 15439 Mouse
- Entrez Gene: 24464 Rat
- Omim: 140100 Human
- SwissProt: P00738 Human
- SwissProt: Q61646 Mouse
- SwissProt: P06866 Rat
- Unigene: 513711 Human
see all -
Alternative names
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha chain antibody
see all
Images
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Anti-Haptoglobin antibody (ab231000) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 37 kDa -
Formalin-fixed, paraffin-embedded rat stomach tissue stained for Haptoglobin with ab231000 at 20 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat testis tissue stained for Haptoglobin with ab231000 at 20 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat liver tissue stained for Haptoglobin with ab231000 at 20 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat spleen tissue stained for Haptoglobin with ab231000 at 20 μg/ml in immunohistochemical analysis.
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Anti-Haptoglobin antibody (ab231000) at 2 µg/ml + Mouse liver lysate
Predicted band size: 37 kDa -
Anti-Haptoglobin antibody (ab231000) at 2 µg/ml + Rat serum
Predicted band size: 37 kDa -
Anti-Haptoglobin antibody (ab231000) at 2 µg/ml + Recombinant rat Haptoglobin
Predicted band size: 37 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231000 has been referenced in 1 publication.
- Tveitarås MK et al. Protein expression profiling of plasma and lungs at different stages of metastatic development in a human triple negative breast cancer xenograft model. PLoS One 14:e0215909 (2019). PubMed: 31042781