Anti-Haptoglobin antibody (ab231013)
Key features and details
- Rabbit polyclonal to Haptoglobin
- Suitable for: WB
- Reacts with: Pig
- Isotype: IgG
Overview
-
Product name
Anti-Haptoglobin antibody
See all Haptoglobin primary antibodies -
Description
Rabbit polyclonal to Haptoglobin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Pig Haptoglobin aa 100 to the C-terminus. (Expressed in E.coli).
Database link: Q8SPS7 -
Positive control
- WB: Pig liver, lung and cerebrum lysates; Pig serum; Recombinant pig Haptoglobin protein.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab231013 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 38 kDa.
|
Notes |
---|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 38 kDa. |
Target
-
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha chain antibody
see all
Images
-
Anti-Haptoglobin antibody (ab231013) at 1 µg/ml + Pig lung lysate
Predicted band size: 38 kDa -
Anti-Haptoglobin antibody (ab231013) at 1 µg/ml + Pig liver lysate
Predicted band size: 38 kDa -
Anti-Haptoglobin antibody (ab231013) at 1 µg/ml + Pig serum
Predicted band size: 38 kDa -
Anti-Haptoglobin antibody (ab231013) at 1 µg/ml + Pig cerebrum lysate
Predicted band size: 38 kDa -
Anti-Haptoglobin antibody (ab231013) at 1 µg/ml + Recombinant pig Haptoglobin protein
Predicted band size: 38 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab231013 has not yet been referenced specifically in any publications.