Overview

  • Product name

    Anti-Haptoglobin antibody [EPSISR7]
    See all Haptoglobin primary antibodies
  • Description

    Rabbit monoclonal [EPSISR7] to Haptoglobin
  • Host species

    Rabbit
  • Specificity

    ab131236 may also recognize Haptoglobin or Haptoglobin-related protein based on the immunogen sequences.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • Positive control

    • Human plasma and fetal liver lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPSISR7
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 45 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
      Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Involvement in disease

      Anhaptoglobinemia
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Binding peptide antibody
      • BP antibody
      • Haptoglobin alpha chain antibody
      • Haptoglobin alpha(1S) beta antibody
      • Haptoglobin alpha(2FS) beta antibody
      • Haptoglobin beta chain antibody
      • Haptoglobin, alpha polypeptide antibody
      • Haptoglobin, beta polypeptide antibody
      • HP antibody
      • HP2 ALPHA2 antibody
      • HP2ALPHA2 antibody
      • HPA1S antibody
      • HPT antibody
      • HPT_HUMAN antibody
      • MGC111141 antibody
      • Zonulin antibody
      see all

    Images

    • All lanes : Anti-Haptoglobin antibody [EPSISR7] (ab131236) at 1/10000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    References

    This product has been referenced in:

    • Kittivorapart J  et al. Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/ß-thalassemic patients. Blood Adv 2:95-104 (2018). Read more (PubMed: 29365317) »
    • Fader KA  et al. Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBa/ß activation in aryl hydrocarbon receptor-elicited hepatotoxicity. Toxicol Appl Pharmacol 321:1-17 (2017). Read more (PubMed: 28213091) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131236.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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