Product nameAnti-Haptoglobin antibody [HG-36]
See all Haptoglobin primary antibodies
DescriptionMouse monoclonal [HG-36] to Haptoglobin
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, Dot blotmore details
Species reactivityReacts with: Human
Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.097% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab13429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Use under non reducing condition.|
|IHC-P||Use at an assay dependent concentration.
|ICC/IF||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseAnhaptoglobinemia
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha chain antibody
ab13429 used in Western Blot.
Tissue lysate was obtained from HOPE-fixed paraffin-embedded blocks of human lung, lung cancer and liver tissue, loaded at 30µg/ml. ab13429 used at a 1/100 dilution. Secondary used was an alkaline phosphatase goat anti-mouse polyclonal used at a 1/10000 dilution.
ab13429 staining Haptoglobin in human bronchoalveolar lavage cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/200 dilution. Counterstained with DAPI.
ab13429 staining Haptoglobin in human lung cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
This product has been referenced in:
- Wu L et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). Read more (PubMed: 28534980) »
- Yang Q et al. iTRAQ-Based Proteomics Investigation of Aqueous Humor from Patients with Coats' Disease. PLoS One 11:e0158611 (2016). WB . Read more (PubMed: 27416065) »