Overview

  • Product name
    Anti-Haptoglobin antibody [HG-36]
    See all Haptoglobin primary antibodies
  • Description
    Mouse monoclonal [HG-36] to Haptoglobin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IF, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma).

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituent: Ascites
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    HG-36
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

See Abreview.

ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

Target

  • Function
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Anhaptoglobinemia
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Binding peptide antibody
    • BP antibody
    • Haptoglobin alpha chain antibody
    • Haptoglobin alpha(1S) beta antibody
    • Haptoglobin alpha(2FS) beta antibody
    • Haptoglobin beta chain antibody
    • Haptoglobin, alpha polypeptide antibody
    • Haptoglobin, beta polypeptide antibody
    • HP antibody
    • HP2 ALPHA2 antibody
    • HP2ALPHA2 antibody
    • HPA1S antibody
    • HPT antibody
    • HPT_HUMAN antibody
    • MGC111141 antibody
    • Zonulin antibody
    see all

Images

  • ab13429 used in Western Blot.Tissue lysate was obtained from HOPE-fixed paraffin-embedded blocks of human lung, lung cancer and liver tissue, loaded at 30µg/ml. ab13429 used at a 1/100 dilution. Secondary used was an alkaline phosphatase goat anti-mouse polyclonal used at a 1/10000 dilution.

    This image was generated using the ascites version of the product.

    See Abreview

  • ab13429 staining Haptoglobin in human bronchoalveolar lavage cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/200 dilution. Counterstained with DAPI.

    This image was generated using the ascites version of the product.

    See Abreview

  • ab13429 staining Haptoglobin in human lung cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:
  • Mariño-Crespo Ó  et al. Identification of proteins with the CDw75 epitope in human colorectal cancer. Oncol Lett 15:580-587 (2018). Read more (PubMed: 29391890) »
  • Wu L  et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). Read more (PubMed: 28534980) »
See all 10 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH = 8
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 30°C
Fixative
Paraformaldehyde

Dr. Christian Ostalecki

Verified customer

Submitted May 25 2018

Application
Western blot
Sample
Human Tissue lysate - whole (Lung, lung cancer and liver)
Loading amount
30 µg
Specification
Lung, lung cancer and liver
Gel Running Conditions
Reduced Denaturing (4%stacking, 12%resolving)
Blocking step
DIG Wash & Block Buffer, Roche as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 08 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung, lung cancer)
Specification
Lung, lung cancer
Fixative
HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Antigen retrieval step
None
Permeabilization
No
Blocking step
Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 03 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human bronchoalveolar lavage cells mainly macroph)
Specification
human bronchoalveolar lavage cells mainly macroph
Fixative
HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Permeabilization
No
Blocking step
Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 03 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver,spleen)
Specification
liver,spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
H²O²/Methanol as blocking agent for 15 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Dec 12 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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