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Thank you for your answer.
I have read the information shown at this site:
and I have a critical question after reading this paragraph:
"In most cases, with the possible exception of ascites fluids which
may contain proteases and should be frozen as soon as possible,
storage at 4oC upon receipt of the antibody is acceptable for one to
two weeks, followed by freezing for long-term storage. Again, it is
important to follow the recommendations on the datasheet."
The Anti-Haptoglobin antibody [HG-36] (ab13429) comes as ascitic
fluid. However, in its datasheet nothing is said about the possibility
of containing proteases, so that it should be frozen as soon as
possible. As I didn't know about this problem I kept it for
approximately 26 hours at 4ºC. Moreover, the antibody was shipped
refrigerated, so I don't really know how long it was not frozen.
After running a immunohistochemical test using this antiboy and
observing an unexpected result I'm seriously thinking about the
possibility the antibody could be damaged.
Could you put some light on this matter?
Asked on Feb 09 2012
Thank you for your response and your comment.
The on-line document contains general information/guidelines for our antibodies for different purifications. Each product has specific storage instructions which can be found on the public website (on-line product datasheet).
This antibody can be stored at +4°C short term (1-2 weeks) but we would suggest aliquoting and keeping at -20°C or -80°C for longer period of time.
If you are happy to share some further details of the IHC protocol; I could certainly take a look at it. There may be some tips/optimization I could perhaps offer if it is necessary and appropriate.
It would be appreciated if you could complete the following form (attached as a word document) and attach an image to the response.
I look forward to hearing from you soon and resolving this issue as soon as possible.
Answered on Feb 09 2012