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I think it was a backgrond signal problem. I was used to obtaining a
high background signal, so when I got such clean slides and I learnt
about that this product can contain proteasas I logically got
concerned about the antibody. So I think the antibody is in good
But, anyway, what about this general paragraph?:
"In most cases, with the possible exception of ascites fluids which
may contain proteases and should be frozen as soon as possible,
storage at 4oC upon receipt of the antibody is acceptable for one to
two weeks, followed by freezing for long-term storage. Again, it is
important to follow the recommendations on the datasheet."
There is a contradiction between that paragraph and this one:
"This antibody can be stored at +4°C short term (1-2 weeks) but we
would suggest aliquoting and keeping at -20°C or -80°C for longer
period of time."
The logic conclusion is that if I keep an antibody coming as ascitic
fluid and cointaining proteases at +4ºC, as the datasheet says,
proteases could digest the antibody.
Asked on Feb 14 2012
Thank you for your email.
Generally antibodies are stable for 1 year at 4C and for 10 years at -20C or -80C. We however recommend storing the antibodies at 4C for short term (1-2 weeks) because some antibodies lose activity if they kept at 4C for longer period of time. We unfortunately can't run stability test for each >70,000 antibody products available in catalogue. We however do experiments for checking the stability with a range of antibodies every year. The data is available on request. Based on the data we have, we choose the statement which is best suitable for all of the products.
Finally, I suggest not to worry about the stability; the product is guaranteed for 6 months if stored as recommended on the datasheet.
I hope this info will be helpful.
Answered on Feb 14 2012