Immunocytochemistry/ Immunofluorescence abreview for Anti-Haptoglobin antibody [HG-36]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human bronchoalveolar lavage cells mainly macroph)
Specification
human bronchoalveolar lavage cells mainly macroph
Fixative
HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Permeabilization
No
Blocking step
Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Zytomed Systems Antibody Diluent

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse Alexa Fluor 488,
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

PubMed ID
21860221: View
Additional Notes
Immunofluorescence staining was performed on HOPE-fixed BAL using primary monoclonal anti-human Hp antibody secondary antibody was labeled with FITC. Nuclei were stained with DAPI.
Image at 400x.

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Submitted Nov 03 2011

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