Western blot abreview for Anti-Haptoglobin antibody [HG-36]

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Lung, lung cancer and liver)
Loading amount
30 µg
Specification
Lung, lung cancer and liver
Gel Running Conditions
Reduced Denaturing (4%stacking, 12%resolving)
Blocking step
DIG Wash & Block Buffer, Roche as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TBSt with 3%non-fat dry milk

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/10000

Detection

Detection method
NBT/BCIP
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 40 kDa

Additional data

PubMed ID
19501987: View
Additional Notes
Tissue liysate was obtained from HOPE-fixed paraffin-embedded blocks.

Abcam user community

Verified customer

Submitted Nov 08 2011

Sign up