Anti-Haptoglobulin beta antibody (ab126842)
Key features and details
- Goat polyclonal to Haptoglobulin beta
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Haptoglobulin beta antibody
See all Haptoglobulin beta primary antibodies -
Description
Goat polyclonal to Haptoglobulin beta -
Host species
Goat -
Tested Applications & Species
Application Species WB HumanPig -
Immunogen
Synthetic peptide:
C-STVPEKKTPKSP
(with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1). -
Positive control
- Human Liver lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
Pig
|
All applications |
Dog
Chimpanzee
Baboon
Macaque monkey
Gorilla
Common marmoset
Orangutan
Bat
|
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100137581 Baboon
- Entrez Gene: 454233 Chimpanzee
- Entrez Gene: 479668 Dog
- Entrez Gene: 3240 Human
- Entrez Gene: 706640 Macaque monkey
- Entrez Gene: 100173782 Orangutan
- Entrez Gene: 397061 Pig
- Omim: 140100 Human
see all -
Alternative names
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha(1S) beta antibody
see all
Images
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Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa -
Western blot - Anti-Haptoglobulin beta antibody (ab126842)This image is courtesy of an anonymous AbreviewLanes 2-3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution
Lane 1 : Marker
Lanes 2-3 : Pig endometrial tissue lysate - whole at 30 µg
Secondary
Lanes 2-3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa (possible non-specific binding), 52 kDa (possible non-specific binding)
Exposure time: 24 minutes52kDa band is from the secondary antibody.
Datasheets and documents
References (0)
ab126842 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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