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    haptoglobulin-beta-antibody-ab126842.pdf

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Anti-Haptoglobulin beta antibody (ab126842)

  • Datasheet
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Western blot - Anti-Haptoglobulin beta antibody (ab126842)
  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)

Key features and details

  • Goat polyclonal to Haptoglobulin beta
  • Suitable for: WB
  • Reacts with: Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Haptoglobin antibody [EPR22856-212] (ab256454)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Haptoglobulin beta antibody
    See all Haptoglobulin beta primary antibodies
  • Description

    Goat polyclonal to Haptoglobulin beta
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    WB
    Human
    Pig
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    C-STVPEKKTPKSP

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human Liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Other

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Haptoglobulin beta protein (ab63120)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Pig
All applications
Dog
Chimpanzee
Baboon
Macaque monkey
Gorilla
Common marmoset
Orangutan
Bat
Application Abreviews Notes
WB (1)
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa.

1 hour primary incubation is recommended for this product.

Notes
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa.

1 hour primary incubation is recommended for this product.

Target

  • Function

    Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P00738 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 100137581 Baboon
    • Entrez Gene: 454233 Chimpanzee
    • Entrez Gene: 479668 Dog
    • Entrez Gene: 3240 Human
    • Entrez Gene: 706640 Macaque monkey
    • Entrez Gene: 100173782 Orangutan
    • Entrez Gene: 397061 Pig
    • Omim: 140100 Human
    • SwissProt: P00738 Human
    • Unigene: 513711 Human
    • Unigene: 708058 Human
    see all
  • Alternative names

    • Binding peptide antibody
    • BP antibody
    • Haptoglobin alpha(1S) beta antibody
    • Haptoglobin alpha(2FS) beta antibody
    • Haptoglobin antibody
    • Haptoglobin beta chain antibody
    • Haptoglobin beta polypeptide antibody
    • Haptoglobin, alpha polypeptide antibody
    • HP antibody
    • HP2ALPHA2 antibody
    • HPA1S antibody
    • HPT_HUMAN antibody
    • MGC111141 antibody
    • Zonulin antibody
    see all

Images

  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate

    Predicted band size: 45 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Western blot - Anti-Haptoglobulin beta antibody (ab126842)This image is courtesy of an anonymous Abreview
    Lanes 2-3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution

    Lane 1 : Marker
    Lanes 2-3 : Pig endometrial tissue lysate - whole at 30 µg

    Secondary
    Lanes 2-3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?
    Additional bands at: 27 kDa (possible non-specific binding), 52 kDa (possible non-specific binding)


    Exposure time: 24 minutes


    52kDa band is from the secondary antibody.

    See Abreview

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab126842? Please let us know so that we can cite the reference in this datasheet.

    ab126842 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Haptoglobulin beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Sample
    Pig Tissue lysate - whole (Porcine endometrial tissue lysate)
    Specification
    Porcine endometrial tissue lysate
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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