Anti-Haptoglobulin beta antibody (ab126842)

Overview

  • Product name
    Anti-Haptoglobulin beta antibody
    See all Haptoglobulin beta primary antibodies
  • Description
    Goat polyclonal to Haptoglobulin beta
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Dog, Chimpanzee, Baboon, Macaque monkey, Gorilla, Common marmoset, Orangutan, Bat
  • Immunogen

    Synthetic peptide:

    C-STVPEKKTPKSP

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1).

  • Positive control
    • Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa.

Target

Images

  • Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate

    Predicted band size: 45 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Lanes 2-3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution

    Lane 1 : Marker
    Lanes 2-3 : Pig endometrial tissue lysate - whole at 30 µg

    Secondary
    Lanes 2-3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 27 kDa (possible non-specific binding), 52 kDa (possible non-specific binding)


    Exposure time: 24 minutes


    52kDa band is from the secondary antibody.

    See Abreview

References

ab126842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Pig Tissue lysate - whole (Porcine endometrial tissue lysate)
Specification
Porcine endometrial tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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