- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Haptoglobulin beta antibody
See all Haptoglobulin beta primary antibodies -
DescriptionGoat polyclonal to Haptoglobulin beta
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Host speciesGoat
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human, Pig
Predicted to work with: Dog, Chimpanzee, Baboon, Macaque monkey, Gorilla, Common marmoset, Orangutan, Bat
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Immunogen
Synthetic peptide:
C-STVPEKKTPKSP
(with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1). -
Positive control
- Human Liver lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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FunctionHaptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
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Tissue specificityExpressed by the liver and secreted in plasma.
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Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 100137581 Baboon
- Entrez Gene: 454233 Chimpanzee
- Entrez Gene: 479668 Dog
- Entrez Gene: 3240 Human
- Entrez Gene: 706640 Macaque monkey
- Entrez Gene: 100173782 Orangutan
- Entrez Gene: 397061 Pig
- Omim: 140100 Human
see all -
Alternative names
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha(1S) beta antibody
see all
Images
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Western blot - Anti-Haptoglobulin beta antibody (ab126842)Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate
Predicted band size: 45 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-Haptoglobulin beta antibody (ab126842)Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa -
Western blot - Anti-Haptoglobulin beta antibody (ab126842)This image is courtesy of an anonymous AbreviewLanes 2-3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution
Lane 1 : Marker
Lanes 2-3 : Pig endometrial tissue lysate - whole at 30 µg
Secondary
Lanes 2-3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 39 kDa (why is the actual band size different from the predicted?)
Additional bands at: 27 kDa (possible non-specific binding), 52 kDa (possible non-specific binding)
Exposure time: 24 minutes52kDa band is from the secondary antibody.
Protocols
References
ab126842 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"