Anti-Haptoglobulin beta antibody (ab126842)

Reviews (1) Submit a question

Overview

  • Product name

    Anti-Haptoglobulin beta antibody
    See all Haptoglobulin beta primary antibodies

  • Description

    Goat polyclonal to Haptoglobulin beta

  • Host species

    Goat

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Dog, Chimpanzee, Baboon, Macaque monkey, Gorilla, Common marmoset, Orangutan, Bat

  • Immunogen

    Synthetic peptide:

    C-STVPEKKTPKSP

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Liver lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa.

Target

Images

  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate

    Predicted band size: 45 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Western blot - Anti-Haptoglobulin beta antibody (ab126842)
    Western blot - Anti-Haptoglobulin beta antibody (ab126842)This image is courtesy of an anonymous Abreview
    Lanes 2-3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution

    Lane 1 : Marker
    Lanes 2-3 : Pig endometrial tissue lysate - whole at 30 µg

    Secondary
    Lanes 2-3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?
    Additional bands at: 27 kDa (possible non-specific binding), 52 kDa (possible non-specific binding)


    Exposure time: 24 minutes


    52kDa band is from the secondary antibody.

    See Abreview

References

ab126842 has not yet been referenced specifically in any publications.

Publishing research using ab126842? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Haptoglobulin beta antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Pig Tissue lysate - whole (Porcine endometrial tissue lysate)
Specification
Porcine endometrial tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com