Overview

  • Product name

  • Description

    Rabbit polyclonal to HARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-286 of Human HARS (Uniprot: P12081).

  • Positive control

    • HepG2 and HCT116 whole cell lysates; Human Hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)

Target

  • Tissue specificity

    Brain, heart, liver and kidney.
  • Involvement in disease

    Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytoplasmic antibody
    • EC 6.1.1.21 antibody
    • FLJ20491 antibody
    • HARS antibody
    • HisRS antibody
    • Histidine tRNA ligase, cytoplasmic antibody
    • histidine translase antibody
    • Histidine tRNA ligase antibody
    • Histidine--tRNA ligase antibody
    • Histidyl tRNA synthetase antibody
    • Histidyl-tRNA synthetase antibody
    • HRS antibody
    • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
    • SYHC_HUMAN antibody
    • USH3B antibody
    see all

Images

  • All lanes : Anti-HARS antibody (ab137591) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labelling HARS with ab137591 at 1/500 dilution.
  • Anti-HARS antibody (ab137591) at 1/500 dilution + HCT116
    whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE

References

ab137591 has not yet been referenced specifically in any publications.

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