Anti-HARS antibody (ab137591)
Key features and details
- Rabbit polyclonal to HARS
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-HARS antibody
See all HARS primary antibodies -
Description
Rabbit polyclonal to HARS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-286 of Human HARS (Uniprot: P12081).
-
Positive control
- HepG2 and HCT116 whole cell lysates; Human Hepatoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
Target
-
Tissue specificity
Brain, heart, liver and kidney. -
Involvement in disease
Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 510937 Cow
- Entrez Gene: 3035 Human
- Omim: 142810 Human
- SwissProt: Q2KI84 Cow
- SwissProt: P12081 Human
- Unigene: 528050 Human
-
Alternative names
- cytoplasmic antibody
- EC 6.1.1.21 antibody
- FLJ20491 antibody
see all
Images
-
All lanes : Anti-HARS antibody (ab137591) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labelling HARS with ab137591 at 1/500 dilution.
-
Anti-HARS antibody (ab137591) at 1/500 dilution + HCT116
whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab137591 has not yet been referenced specifically in any publications.