Key features and details
- Rabbit polyclonal to HARS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-HARS antibody
See all HARS primary antibodies
DescriptionRabbit polyclonal to HARS
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
- WB: Caco-2, NIH/3T3 and NBT-II whole cell lysates. IHC-P: Human skin, cerebral cortex, cerebellum and kidney tissue. ICC/IF: A431 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 57 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Tissue specificityBrain, heart, liver and kidney.
Involvement in diseaseDefects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
Contains 1 WHEP-TRS domain.
- Information by UniProt
- cytoplasmic antibody
- EC 18.104.22.168 antibody
- FLJ20491 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for HARS (green) using ab246996 at 4 μg/ml in ICC/IF.
All lanes : Anti-HARS antibody (ab246996) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) transfected with control siRNA, whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) transfected with target-specific siRNA #1, whole cell lysate
Lane 3 : Caco-2 (human colorectal adenocarcinoma cell line) transfected with target-specific siRNA #2, whole cell lysate
Predicted band size: 57 kDa
Loading control: Anti-GAPDH.
Paraffin-embedded human kidney tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebellum tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human skin tissue stained for HARS using ab246996 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-HARS antibody (ab246996) at 0.4 µg
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 57 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246996 has not yet been referenced specifically in any publications.