Recombinant
RabMAb

Recombinant Anti-HARS antibody [EPR9450(B)(ABC)] (ab140640)

Overview

  • Product name

    Anti-HARS antibody [EPR9450(B)(ABC)]
    See all HARS primary antibodies
  • Description

    Rabbit monoclonal [EPR9450(B)(ABC)] to HARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HARS aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human HARS protein (ab99387) can be used as a positive control in WB. HeLa, 293T and K562 cell lysates; Human kidney tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 oC, below boiling, and then let cool for 10-20 min
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Tissue specificity

      Brain, heart, liver and kidney.
    • Involvement in disease

      Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
    • Sequence similarities

      Belongs to the class-II aminoacyl-tRNA synthetase family.
      Contains 1 WHEP-TRS domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • cytoplasmic antibody
      • EC 6.1.1.21 antibody
      • FLJ20491 antibody
      • HARS antibody
      • HisRS antibody
      • Histidine tRNA ligase, cytoplasmic antibody
      • histidine translase antibody
      • Histidine tRNA ligase antibody
      • Histidine--tRNA ligase antibody
      • Histidyl tRNA synthetase antibody
      • Histidyl-tRNA synthetase antibody
      • HRS antibody
      • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
      • SYHC_HUMAN antibody
      • USH3B antibody
      see all

    Images

    • All lanes : Anti-HARS antibody [EPR9450(B)(ABC)] (ab140640) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat-anti-Rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HARS with ab140640 at 1/50 dilution.

    References

    ab140640 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140640.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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