Anti-HAS1 antibody (ab198846)
Key features and details
- Rabbit polyclonal to HAS1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HAS1 antibody -
Description
Rabbit polyclonal to HAS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HAS1 (internal sequence). The exact sequence is proprietary.
Database link: Q92839 -
Positive control
- Human testis and prostate tissue lysates, Human thyroid cancer tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/30 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 65 kDa.
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Notes |
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IHC-P
1/30 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 65 kDa. |
Target
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Function
Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate. -
Tissue specificity
Widely expressed. Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine. -
Pathway
Glycan biosynthesis; hyaluronan biosynthesis. -
Sequence similarities
Belongs to the NodC/HAS family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3036 Human
- Omim: 601463 Human
- SwissProt: Q92839 Human
- Unigene: 57697 Human
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Alternative names
- HA synthase 1 antibody
- HAS antibody
- HAS1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling HAS1 with ab198846 at 1/30.
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All lanes : Anti-HAS1 antibody (ab198846) at 1/350 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human prostate tissue lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 30 secondsGel: 6%SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab198846 has been referenced in 3 publications.
- Liao HX et al. CircHYBID regulates hyaluronan metabolism in chondrocytes via hsa-miR-29b-3p/TGF-ß1 axis. Mol Med 27:56 (2021). PubMed: 34058990
- Qi S et al. P38 MAPK signaling pathway mediates COM crystal-induced crystal adhesion change in rat renal tubular epithelial cells. Urolithiasis 48:9-18 (2020). PubMed: 31183507
- Knudtson JF et al. The Hyaluronic Acid System is Intact in Menstrual Endometrial Cells in Women With and Without Endometriosis. Reprod Sci 26:109-113 (2019). PubMed: 29621955