Overview

  • Product name

  • Description

    Rabbit polyclonal to Haspin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide within Human Haspin aa 300-350. The exact sequence is proprietary. (NP_114171.2).
    Database link: Q8TF76

  • Positive control

    • IHC-P: Human prostate carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab117791 was affinity purified using an epitope specific to Haspin immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Target

  • Function

    Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis.
  • Tissue specificity

    Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • germ cell associated 2 antibody
    • Germ cell-specific gene 2 protein antibody
    • GSG2 antibody
    • H-haspin antibody
    • Haploid germ cell-specific nuclear protein kinase antibody
    • HASP_HUMAN antibody
    • Serine/threonine-protein kinase haspin antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Haspin using ab117791 at 1/250 dilution in immunohistochemical analysis. Detection: DAB staining.

References

ab117791 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton in PBS
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 18 2014

Question
Answer

Thank you for your enquiry. Unfortunately, we have yet to test this product in mouse species. I have taken the liberty of running a BLAST analysis and according to the results, there is only 46% homology with mouse species. We can usually expect positive reactivity if the homology is more than 90% but having a low homology percentage does not necessary mean no cross reactivity whatsoever. This is simply a way to check for suitability. I hope this information will be helpful.

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