Overview

  • Product name

    Anti-HAUS3 antibody
  • Description

    Rabbit polyclonal to HAUS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HAUS3 aa 75-125. The exact sequence is proprietary.
    Database link: Q68CZ6

  • Positive control

    • IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use at 8 - 15 µl/mg lysate.

Target

  • Function

    Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
  • Sequence similarities

    Belongs to the HAUS3 family.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Localizes to interphase centrosomes and to mitotic spindle microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • C4orf15 antibody
    • Chromosome 4 open reading frame 15 antibody
    • dgt3 antibody
    • HAUS augmin-like complex subunit 3 antibody
    • haus3 antibody
    • HAUS3_HUMAN antibody
    • IT1 antibody
    • MGC4701 antibody
    see all

Images

  • HAUS3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228822 at 8 µl per reaction. Western blot was performed from the immunoprecipitate using ab228822 at a 1/1000 dilution.

    Lane 1: ab228822 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:Chemiluminescence with exposure time of 3 minutes.

References

ab228822 has not yet been referenced specifically in any publications.

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