• Product name

    Anti-HAUSP / USP7 antibody
    See all HAUSP / USP7 primary antibodies
  • Description

    Rabbit polyclonal to HAUSP / USP7
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment corresponding to Human HAUSP/ USP7 aa 1-300.


    Database link: Q93009

  • Positive control

    • U20S cell lysate, Mouse embrio fibroblasts cell lysate, INS1 cell lysate and DT40 cell lysate



Our Abpromise guarantee covers the use of ab190183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 128 kDa.
ICC/IF 1/500.


  • Function

    Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).
  • Tissue specificity

    Widely expressed. Overexpressed in prostate cancer.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 1 MATH domain.
    Contains 1 USP domain.
  • Domain

    The C-terminus plays a role in its oligomerization.
  • Post-translational

    Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
    Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
    Isoform 1 and isoform 2: Not sumoylated.
    Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 7 antibody
    • HAUSP antibody
    • Herpes virus associated ubiquitin specific protease antibody
    • Herpesvirus-associated ubiquitin-specific protease antibody
    • TEF 1 antibody
    • tef-1 antibody
    • TEF1 antibody
    • Ubiquitin carboxyl terminal hydrolase 7 antibody
    • Ubiquitin carboxyl-terminal hydrolase 7 antibody
    • Ubiquitin specific peptidase 7 (herpes virus associated) antibody
    • Ubiquitin specific peptidase 7 antibody
    • Ubiquitin specific peptidase 7 herpes virus associated antibody
    • Ubiquitin specific processing protease 7 antibody
    • Ubiquitin specific protease 7 (herpes virus associated) antibody
    • Ubiquitin specific protease 7 antibody
    • Ubiquitin specific protease 7 herpes virus associated antibody
    • Ubiquitin thioesterase 7 antibody
    • Ubiquitin thiolesterase 7 antibody
    • Ubiquitin-specific-processing protease 7 antibody
    • UBP 7 antibody
    • UBP-7 antibody
    • UBP7 antibody
    • UBP7_HUMAN antibody
    • USP 7 antibody
    • usp-7 antibody
    • Usp7 antibody
    • VMW110-ASSOCIATED PROTEIN, 135-KD antibody
    see all


  • Immunofluorescent analysis of U2OS cells labeling HAUSP / USP7 (green) with ab190183 at a dilution of 1/500 (merged with DAPI signal on the right panels) . Top panels: U2OS treated with control siRNA, botton panels: U2Os treated with siRNA against HAUSP / USP7.

  • All lanes : Anti-HAUSP / USP7 antibody (ab190183) at 1/2000 dilution

    Lane 1 : U2OS cell lysate si control
    Lane 2 : U2OS cells transfected with HAUSP / USP7 siRNA
    Lane 3 : Mouse embrio fibroblasts cell lysate
    Lane 4 : INS1 cell lysate
    Lane 5 : DT40 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 128 kDa

  • All lanes : Anti-HAUSP / USP7 antibody (ab190183) at 1/2000 dilution

    Lane 1 : Xenopus laevis crude egg extracts
    Lane 2 : U2OS whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 128 kDa


This product has been referenced in:

  • Episkopou H  et al. TSPYL5 Depletion Induces Specific Death of ALT Cells through USP7-Dependent Proteasomal Degradation of POT1. Mol Cell 75:469-482.e6 (2019). WB ; Human . Read more (PubMed: 31278054) »
  • Hernández-Pérez S  et al. DUB3 and USP7 de-ubiquitinating enzymes control replication inhibitor Geminin: molecular characterization and associations with breast cancer. Oncogene N/A:N/A (2017). WB ; Human . Read more (PubMed: 28288134) »
See all 3 Publications for this product

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