• Product name

    Anti-HAUSP / USP7 antibody
    See all HAUSP / USP7 primary antibodies
  • Description

    Goat polyclonal to HAUSP / USP7
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Rhesus monkey, Common marmoset, Zebra finch, Bat
  • Immunogen

    Synthetic peptide; corresponding to a region between residues 1-50 of Human HAUSP/USP7 (NP_003461.1).

  • Positive control

    • 293T, HeLa, Jurkat, NIH 3T3, Renca, and TCMK1 whole cell lysates.



Our Abpromise guarantee covers the use of ab157132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/1000. Predicted molecular weight: 128 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).
  • Tissue specificity

    Widely expressed. Overexpressed in prostate cancer.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 1 MATH domain.
    Contains 1 USP domain.
  • Domain

    The C-terminus plays a role in its oligomerization.
  • Post-translational

    Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
    Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
    Isoform 1 and isoform 2: Not sumoylated.
    Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 7 antibody
    • HAUSP antibody
    • Herpes virus associated ubiquitin specific protease antibody
    • Herpesvirus-associated ubiquitin-specific protease antibody
    • TEF 1 antibody
    • tef-1 antibody
    • TEF1 antibody
    • Ubiquitin carboxyl terminal hydrolase 7 antibody
    • Ubiquitin carboxyl-terminal hydrolase 7 antibody
    • Ubiquitin specific peptidase 7 (herpes virus associated) antibody
    • Ubiquitin specific peptidase 7 antibody
    • Ubiquitin specific peptidase 7 herpes virus associated antibody
    • Ubiquitin specific processing protease 7 antibody
    • Ubiquitin specific protease 7 (herpes virus associated) antibody
    • Ubiquitin specific protease 7 antibody
    • Ubiquitin specific protease 7 herpes virus associated antibody
    • Ubiquitin thioesterase 7 antibody
    • Ubiquitin thiolesterase 7 antibody
    • Ubiquitin-specific-processing protease 7 antibody
    • UBP 7 antibody
    • UBP-7 antibody
    • UBP7 antibody
    • UBP7_HUMAN antibody
    • USP 7 antibody
    • usp-7 antibody
    • Usp7 antibody
    • VMW110-ASSOCIATED PROTEIN, 135-KD antibody
    see all


  • All lanes : Anti-HAUSP / USP7 antibody (ab157132) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 128 kDa

    Exposure time: 3 minutes
  • All lanes : Anti-HAUSP / USP7 antibody (ab157132) at 0.4 µg

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : Renca whole cell lysate
    Lane 3 : TCMK 1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 128 kDa

    Exposure time: 30 seconds
  • Detection of HAUSP / USP7 by Western Blot of Immunprecipitate.
    anti-HAUSP / USP7 antibody at 1µg/ml labeling HAUSP / USP7 in 293T whole cell lysate immunoprecipitated using ab157132 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.


ab157132 has not yet been referenced specifically in any publications.

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