Anti-HAX1 antibody (ab137613)
Key features and details
- Rabbit polyclonal to HAX1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HAX1 antibody
See all HAX1 primary antibodies -
Description
Rabbit polyclonal to HAX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human HAX1 aa 1-279.
Database link: O00165 -
Positive control
- WB: MOLT-4, Raji whole cell lysate. HEK-293T, A431, HeLa, HepG2 whole cell extracts. IHC-P: NCIN87 Xenograft. Rat ovary. ICC/IF: A431.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools. -
Tissue specificity
Ubiquitous. Up-regulated in oral cancers. -
Involvement in disease
Defects in HAX1 are the cause of neutropenia severe congenital autosomal recessive type 3 (SCN3) [MIM:610738]; also known as Kostmann disease. A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Some patients affected by severe congenital neutropenia type 3 have neurological manifestations such as psychomotor retardation and seizures. Note=The clinical phenotype due to HAX1 deficiency appears to depend on the localization of the mutations and their influence on the transcript variants. Mutations affecting exclusively isoform 1 are associated with isolated congenital neutropenia, whereas mutations affecting both isoform 1 and isoform 5 are associated with additional neurologic symptoms. -
Sequence similarities
Belongs to the HAX1 family. -
Post-translational
modificationsProteolytically cleaved by caspase-3 during apoptosis. -
Cellular localization
Mitochondrion. Endoplasmic reticulum. Nucleus membrane. Cytoplasmic vesicle. Sarcoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 10456 Human
- Entrez Gene: 291202 Rat
- Omim: 605998 Human
- SwissProt: O00165 Human
- SwissProt: Q7TSE9 Rat
- Unigene: 199625 Human
- Unigene: 185269 Rat
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Alternative names
- FLJ17042 antibody
- FLJ18492 antibody
- FLJ93803 antibody
see all
Images
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All lanes : Anti-HAX1 antibody (ab137613) at 1/1000 dilution
Lane 1 : MOLT-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS PAGE -
ab137613 at a 1/500 dilution, staining HAX1 in methanol-fixed A431 cells by Immunofluorescence (green).
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ab137613, at a 1/100 dilution, staining HAX1 in paraffin embedded NCIN87 xenograft tissue by Immunohistochemistry.
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All lanes : Anti-HAX1 antibody (ab137613) at 1/1000 dilution
Lane 1 : HEK-293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa12% SDS-PAGE
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Paraffin embedded rat ovary tissue stained for HAX1 using ab137613 at 1/500 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab137613 has been referenced in 6 publications.
- Xia X et al. Effect of different expression patterns of HAX-1 on the proliferation and apoptosis of human astrocyte. Adv Clin Exp Med 31:689-699 (2022). PubMed: 35302298
- Cupo RR & Shorter J Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations. Elife 9:N/A (2020). PubMed: 32573439
- Huang F et al. miR-654-5p Targets HAX-1 to Regulate the Malignancy Behaviors of Colorectal Cancer Cells. Biomed Res Int 2020:4914707 (2020). PubMed: 32104694
- Zha Z & Li J MicroRNA-125a-5p regulates liver cancer cell growth, migration and invasion and EMT by targeting HAX1. Int J Mol Med 46:1849-1861 (2020). PubMed: 33000203
- Wu Z et al. Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt. Cancer Biol Ther 20:1223-1233 (2019). PubMed: 31132019
- Mazel-Sanchez B et al. H5N1 Influenza A Virus PB1-F2 Relieves HAX-1-Mediated Restriction of Avian Virus Polymerase PA in Human Lung Cells. J Virol 92:N/A (2018). PubMed: 29563290